Kebede Muluken, Guadie Demsachew, Kidanemariam Dawit, Abraham Adane
Department of Biotechnology, College of Biological and Chemical Engineering, Addis Ababa Science and Technology University, P.O box 16417, Addis Ababa, Ethiopia.
Institute of Biotechnology, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopia.
Virusdisease. 2023 Jun;34(2):213-220. doi: 10.1007/s13337-023-00816-z. Epub 2023 Apr 15.
The productivity of cabbage ( var in Ethiopia has been generally low due to several biotic and abiotic constraints among which are several viral diseases. There is a recent report indicating that this economically important vegetable is seriously affected in Ethiopia by cauliflower mosaic virus (CaMV) and turnip mosaic virus (TuMV). However, little information exists on the incidence and distribution of these viruses as the previous report is based on samples only from Addis Ababa. In this study, a total of 370 leaf samples were collected from 75 cabbage growing fields in Central Ethiopia in two rounds of survey. Two cabbage varieties locally known as " and with virus-like symptoms were collected and tested with Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) using polyclonal antibodies specific to CaMV and TuMV. Results from serological diagnosis were confirmed with PCR and Sanger sequencing. The results indicated a high incidence and wide distribution of both viruses in Central Ethiopia with an average of 29.5% infection for CaMV and 40% for TuMV. Biological inoculation tests for CaMV or TuMV or both on healthy cabbage seedlings gave similar symptoms as those observed in the field. Symptom severity was higher with co-infection of CaMV and TuMV followed by TuMV single infection. BLAST analysis showed that TuMV and CaMV isolates from Ethiopia have nucleotide identity of 95-98% and 93-98%, respectively to previously reported isolates. Phylogenetic analysis revealed that CaMV isolates from Ethiopia are closely related to isolates from USA and Italy within Group II clade whereas TuMV isolates have close similarities with isolates from World B clade including isolates from Kenya, UK, Japan and the Netherlands. The identification of the causative agents of the mosaic disease observed on cabbage in Central Ethiopia may lay the foundation for future management studies.
由于多种生物和非生物限制因素，埃塞俄比亚的甘蓝（品种）产量普遍较低，其中包括几种病毒性疾病。最近有报告表明，这种具有重要经济价值的蔬菜在埃塞俄比亚受到花椰菜花叶病毒（CaMV）和芜菁花叶病毒（TuMV）的严重影响。然而，关于这些病毒的发病率和分布情况的信息很少，因为之前的报告仅基于亚的斯亚贝巴的样本。在本研究中，在两轮调查中从埃塞俄比亚中部75个甘蓝种植田收集了总共370份叶片样本。收集了两个当地称为“ ”和 且有病毒样症状的甘蓝品种，并使用针对CaMV和TuMV的多克隆抗体通过双抗体夹心酶联免疫吸附测定（DAS-ELISA）进行检测。血清学诊断结果通过PCR和桑格测序得到证实。结果表明，这两种病毒在埃塞俄比亚中部发病率高且分布广泛，CaMV平均感染率为29.5%，TuMV为40%。对健康甘蓝幼苗进行CaMV或TuMV或两者的生物接种试验产生了与田间观察到的相似症状。CaMV和TuMV共同感染时症状严重程度更高，其次是TuMV单一感染。BLAST分析表明，埃塞俄比亚的TuMV和CaMV分离株与先前报道的分离株的核苷酸同一性分别为95 - 98%和93 - 98%。系统发育分析表明，埃塞俄比亚的CaMV分离株与第二组进化枝内来自美国和意大利的分离株密切相关，而TuMV分离株与来自世界B进化枝的分离株密切相似，包括来自肯尼亚、英国、日本和荷兰的分离株。确定埃塞俄比亚中部甘蓝上观察到的花叶病病原体可能为未来的管理研究奠定基础。
