Kabra S K, Kabra M, Gera S, Lodha R, Sreedevi K N, Chacko S, Mathew J, Shastri S, Ghosh M
Department of Pediatrics, All India Institute of Medical Sciences, New Delhi 110 029, India.
Indian Pediatr. 2002 Nov;39(11):1039-43.
An indigenously developed method for sweat collection and titration method for estimation of chloride was validated. The mean difference in estimated chloride value from the known strength of saline in 50 samples was -1.04 +/- 4.13 mEq/L (95% CI: -0.07 to 2.28). The mean difference in the estimated chloride values between two observers when the test was performed on known strengths of saline solution was -2.5 +/- 4.24 mEq/L (95% CI: -3.67 to 1.33). The inter observer variability between two observers when the test was performed on sweat samples obtained from 50 individuals was -1.12 +/- 4.34 mEq/L (95% CI: -2.23 to 0.8 ). Sweat weight of more than 100 mg could be collected in first attempt in 602 of 757 (80%) patient with an average sweat weight of 230 mg. This inexpensive method of sweat collection and chloride estimation has acceptable accuracy and repeatability and can be used in resource poor setting for making a diagnosis of cystic fibrosis.
一种自主研发的汗液采集方法和用于氯化物估算的滴定法得到了验证。50个样本中，根据已知浓度盐水估算的氯化物值的平均差异为-1.04 +/- 4.13 mEq/L（95%置信区间：-0.07至2.28）。对已知浓度的盐溶液进行测试时，两名观察者估算的氯化物值的平均差异为-2.5 +/- 4.24 mEq/L（95%置信区间：-3.67至1.33）。对从50名个体采集的汗液样本进行测试时，两名观察者之间的观察者间变异性为-1.12 +/- 4.34 mEq/L（95%置信区间：-2.23至0.8）。757例患者中有602例（80%）在首次尝试时就能采集到超过100 mg的汗液，平均汗液重量为230 mg。这种廉价的汗液采集和氯化物估算方法具有可接受的准确性和可重复性，可用于资源匮乏地区的囊性纤维化诊断。
