Tecirlioğlu R T, Hayes E S, Trounson A O
Centre for Early Human Development, Monash Institute of Reproduction and Development, Clayton, Victoria, Australia.
Reprod Fertil Dev. 2002;14(5-6):363-71. doi: 10.1071/rd02015.
The effects of device type (electrostimulator, function generator or computer-generated waveforms), waveform (square, triangle or sine wave), probe type (ring or strip) and anaesthetic compound (ketamine/xylazine combination or pentobarbitone sodium) were investigated on electroejaculation (EEJ) responses of C57B1 x CBA and C57Bl/6J mice. Ejaculates were analysed for total sperm count and motility variables using computer-assisted sperm analyses. Automated computer-generated waveforms delivered through a sound card were more effective and reproducible compared with waveforms generated by function generator and electrostimulator. Sine waves and triangle waves were found to be more effective in producing ejaculate than square waves. As an anaesthetic, pentobarbitone sodium tended to outperform ketamine/xylazine across waveforms and strains. Strip probes failed to produce any ejaculate regardless of the device or waveform employed. Sperm obtained by EEJ exhibited poor motility and C5B1/6J mice had lower motility variables than C57BI x CBA mice.
研究了设备类型（电刺激器、函数发生器或计算机生成的波形）、波形（方波、三角波或正弦波）、探头类型（环形或带状）和麻醉剂组合（氯胺酮/赛拉嗪组合或戊巴比妥钠）对C57B1×CBA和C57Bl/6J小鼠电射精（EEJ）反应的影响。使用计算机辅助精子分析对射精样本进行总精子计数和活力变量分析。与函数发生器和电刺激器产生的波形相比，通过声卡传输的自动计算机生成的波形更有效且可重复。发现正弦波和三角波在产生射精方面比方波更有效。作为麻醉剂，戊巴比妥钠在各种波形和品系中往往优于氯胺酮/赛拉嗪。无论使用何种设备或波形，带状探头均未能产生任何射精。通过电射精获得的精子活力较差，且C5B1/6J小鼠的活力变量低于C57BI×CBA小鼠。
