Anderson R A, Oswald C, Willis B R, Zaneveld L J
J Reprod Fertil. 1983 May;68(1):1-7. doi: 10.1530/jrf.0.0680001.
Ejaculates were obtained from C57BL mice by applying two successive series of electrical stimuli which were delivered via a bipolar rectal probe. The ejaculates thus collected contained fertile spermatozoa as indicated by results from in-vitro fertilization. Once separated from the seminal plasma, ejaculated spermatozoa possessed the same in-vitro fertilization rate as epididymal sperm. Ejaculates were analysed for coagulum weight, ejaculate volume, sperm count, sperm motility, acid phosphatase content and fructose content. Significant differences were present between several of these values for fertile and infertile mice, and values were therefore empirically assigned to represent minimal amounts for 'normal' fertility (1.5 microliters ejaculate volume; 10.2 mg coagulum weight; 2.5 x 10(6) spermatozoa/ml; 2.3 x 10(3) motile spermatozoa/ejaculate). One half of the fertile animals had no deficiencies in any of the characteristics measured, whereas 97% of the infertile animals had at least one deficiency. No fertile male had more than 2 deficiencies. These data show that the characteristics of mouse semen obtained by the present method of electroejaculation are related to the fertility status of the animal.
通过经由双极直肠探针施加连续两个系列的电刺激，从C57BL小鼠获取射精样本。如体外受精结果所示，如此收集的射精样本含有可育精子。一旦从精浆中分离出来，射出的精子具有与附睾精子相同的体外受精率。对射精样本进行了凝固物重量、射精量、精子计数、精子活力、酸性磷酸酶含量和果糖含量的分析。可育和不育小鼠的这些值中的几个存在显著差异，因此根据经验确定了代表“正常”生育能力的最小值（射精量1.5微升；凝固物重量10.2毫克；每毫升2.5×10⁶个精子；每次射精2.3×10³个活动精子）。一半的可育动物在所测量的任何特征上都没有缺陷，而97%的不育动物至少有一个缺陷。没有可育雄性有超过2个缺陷。这些数据表明，通过本电射精方法获得的小鼠精液特征与动物的生育状态相关。
