利用蓝光发光二极管（LED）光照进行雨生红球藻生产虾青素的补料分批培养。

Fed-batch culture under illumination with blue light emitting diodes (LEDs) for astaxanthin production by Haematococcus pluvialis.

作者信息

Lababpour Abdolmajid, Shimahara Kazumichi, Hada Keishi, Kyoui Yoshiaki, Katsuda Tomohisa, Katoh Shigeo

机构信息

Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.

出版信息

J Biosci Bioeng. 2005 Sep;100(3):339-42. doi: 10.1263/jbb.100.339.

DOI:10.1263/jbb.100.339

PMID:16243287

Abstract

To increase the cell concentration and the accumulation of astaxanthin, the effects of the fed-batch addition of 10-fold-concentrated medium to supply nutrients, as well as illumination with blue light emitting diodes (LEDs), on cell growth and accumulation of astaxanthin were studied for the cultivation of Haematococcus pluvialis. Using the fed-batch addition method, the cell concentration increased above 1 mg-dry cell/cm3, and under illumination with blue LEDs, the astaxanthin concentration reached approximately 70 microg/cm3. This method was much simpler to operate than the medium replacement method in operation and enabled us to attain a higher total yield of astaxanthin.

摘要

为提高细胞浓度和虾青素的积累量，研究了补料分批添加10倍浓缩培养基以供应营养物质以及用蓝色发光二极管（LED）照明对雨生红球藻培养过程中细胞生长和虾青素积累的影响。采用补料分批添加法，细胞浓度增加到1mg干细胞/cm³以上，在蓝色LED照明下，虾青素浓度达到约70μg/cm³。该方法在操作上比培养基更换法简单得多，并且能使我们获得更高的虾青素总产量。