Suh In Soo, Joo Hyun-Na, Lee Choul-Gyun
Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University, 253 Yonghyun-Dong, Nam-Gu, Incheon 402-751, Republic of Korea.
J Biotechnol. 2006 Oct 1;125(4):540-6. doi: 10.1016/j.jbiotec.2006.03.027. Epub 2006 May 2.
This study proposes a novel double-region photobioreactor to simplify the commercial two-stage process of astaxanthin production by the cultivation of Haematococcus pluvialis. The feasibility of the double-region photobioreactor has been investigated and found to achieve high biomass yield in the inner core region and simultaneous astaxanthin accumulation in the outer jacket region. Among many environmental factors, light condition and nitrate level were manipulated for selective cell growth and astaxanthin production. In the outer jacket region, efficient astaxanthin production was accomplished by excessive irradiation (770+/-20 microE m(-2)s(-1)) and nitrate starvation, resulting in a dramatic increase of astaxanthin productivity (357 mg l(-1)). Meanwhile, attenuated light energy (40+/-3 microE m(-2)s(-1)) and sufficient nitrates were supplied to the vegetative cells in the inner core region, which continued to grow to a high cell concentration of 4.0 x 10(5) cells ml(-1). The sequential batch run was performed by utilizing the high-density vegetative cells as inoculum for the next batch run. The cultivation results exhibited similar trends as the previous run, reaching high cell density (4.3 x 10(5) cells ml(-1)) in the inner core region and high astaxanthin content (5.79% on a dry weight basis) in the outer jacket region. The present study indicates that the double-region photobioreactor and its method of operation possess a good potential for commercial production of astaxanthin by H. pluvialis.
本研究提出了一种新型双区域光生物反应器，以简化通过雨生红球藻培养生产虾青素的商业化两阶段工艺。对双区域光生物反应器的可行性进行了研究，发现其在内核区域实现了高生物量产量，在外套区域同时积累了虾青素。在众多环境因素中，对光照条件和硝酸盐水平进行了调控，以实现选择性细胞生长和虾青素生产。在外套区域，通过过度照射（770±20 μE m(-2)s(-1)）和硝酸盐饥饿实现了高效虾青素生产，导致虾青素生产力显著提高（357 mg l(-1)）。同时，向内核区域的营养细胞提供衰减的光能（40±3 μE m(-2)s(-1)）和充足的硝酸盐，这些细胞继续生长至4.0×10(5) 个细胞 ml(-1)的高细胞浓度。通过利用高密度营养细胞作为下一批次运行的接种物进行了连续批次运行。培养结果呈现出与前一批次相似的趋势，在内核区域达到了高细胞密度（4.3×10(5) 个细胞 ml(-1)），在外套区域达到了高虾青素含量（以干重计为5.79%）。本研究表明，双区域光生物反应器及其操作方法在雨生红球藻商业化生产虾青素方面具有良好的潜力。
