Nemanic Sarah, Alvarado Maria C, Price Roger E, Jackson Edward F, Bachevalier Jocelyne
Department of Neurobiology and Anatomy, University of Texas Health Science Center, 6431 Fannin Street, Houston, TX 77030, USA.
J Neurosci Methods. 2002 Dec 15;121(2):199-209. doi: 10.1016/s0165-0270(02)00264-9.
In a recent study, [Hippocampus 11 (2001) 361] demonstrated that in vivo neuroimaging techniques could be used to accurately quantify the extent of neuronal damage after ibotenic acid injections in non-human primates. The present study was undertaken to replicate these findings and to further estimate whether the concentration of ibotenic acid used (10-15 mg/ml) to produce the neuronal loss did not affect the fibers coursing within or around the targeted brain area. Magnetic resonance (MR) images (T1-weighted and FLAIR) were acquired in three monkeys before and after they received neurotoxic lesions of the hippocampal formation. The postsurgical FLAIR images were taken 7-10 days after surgery to visualize the hyperintense signals produced by increased edema at the injection sites. One year post-surgically, T1-weighted images were acquired and compared with T1-weighted images obtained pre-surgery to estimate reduction in hippocampal volume resulting from neuronal loss. Estimated neuronal loss was then compared with actual cell loss found during histological evaluation of brain tissue. Both neuroimaging techniques accurately estimated the extent of hippocampal damage and damage to surrounding structures. In addition, the concentration of ibotenic acid (10 mg/ml) used in the present study did not appear to have significantly damaged or de-myelinated fibers coursing through or around the hippocampal formation. Together with the previous results of [Hippocampus 11 (2001) 361], the present data strongly demonstrate that in vivo neuroimaging techniques provide powerful tools to estimate reliably and rapidly the extent and localization of brain lesions in non-human primates.
在最近的一项研究中，[《海马体》11(2001)361]表明，体内神经成像技术可用于准确量化在非人灵长类动物中注射鹅膏蕈氨酸后神经元损伤的程度。本研究旨在重复这些发现，并进一步评估用于产生神经元损失的鹅膏蕈氨酸浓度(10 - 15毫克/毫升)是否不会影响在目标脑区内部或周围走行的纤维。在三只猴子接受海马结构的神经毒性损伤之前和之后，获取了磁共振(MR)图像(T1加权和液体衰减反转恢复序列)。术后7 - 10天拍摄液体衰减反转恢复序列图像，以显示注射部位水肿增加产生的高强度信号。术后一年，获取T1加权图像，并与术前获得的T1加权图像进行比较，以评估由于神经元损失导致的海马体积减小。然后将估计的神经元损失与在脑组织组织学评估中发现的实际细胞损失进行比较。两种神经成像技术都准确地估计了海马损伤的程度以及对周围结构的损伤。此外，本研究中使用的鹅膏蕈氨酸浓度(10毫克/毫升)似乎并未对穿过或围绕海马结构走行的纤维造成明显损伤或脱髓鞘。与[《海马体》11(2001)361]之前的结果一起，目前的数据有力地证明，体内神经成像技术为可靠且快速地估计非人灵长类动物脑损伤的程度和定位提供了强大的工具。
