Komorowska Małgorzata, Cuissot Arnaud, Czarnołeski Adam, Białas Wieslaw
Institute of Physics, Wrocław University of Technology, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland.
J Photochem Photobiol B. 2002 Nov;68(2-3):93-100. doi: 10.1016/s1011-1344(02)00361-5.
The effects of NIR (near-infrared radiation 700-2,000 nm) on bovine erythrocytes in plasma was studied as a continuation of earlier studies. Cell shape was observed and the changes of ratio of hemolysis and electrokinetic potential measured as a function of irradiation time. After 10 min of irradiation, the shape of erythrocyte cells was mainly echinocytic. When these cells were incubated at 311 K for 24 h they regained their initial shape, but fresh erythrocytes that were irradiated for 30 min and aged in vitro did not. These phenomena are due to: (1) the absorption of NIR excitation by hemoglobin; the primary photochemical process being the photo-dissociation of oxyhemoglobin to deoxyhemoglobin. Resulting shape and ratio of hemolysis, structural changes and oxidative stress follow higher deoxyhemoglobin concentration. (2) The absorption of the NIR excitation by proteins, water and lipids. After NIR absorption the membrane surface dehydrates, leading to enhanced protonation and dissociation of hydrogen-bonded complexes. This in turn leads to a change in electrokinetic potential.
作为早期研究的延续，我们研究了近红外辐射（700 - 2000纳米）对血浆中牛红细胞的影响。观察细胞形态，并测量溶血率和动电电位随照射时间的变化。照射10分钟后，红细胞的形态主要为棘状细胞。当这些细胞在311K下孵育24小时后，它们恢复了初始形状，但照射30分钟并在体外老化的新鲜红细胞则没有。这些现象是由于：（1）血红蛋白对近红外激发的吸收；主要的光化学过程是氧合血红蛋白光解离为脱氧血红蛋白。溶血的形状和比例、结构变化以及氧化应激随脱氧血红蛋白浓度升高而变化。（2）蛋白质、水和脂质对近红外激发的吸收。近红外吸收后，膜表面脱水，导致氢键复合物的质子化增强和解离。这进而导致动电电位的变化。
