Kujawa Jolanta, Pasternak Kamila, Zavodnik Ilya, Irzmański Robert, Wróbel Dominika, Bryszewska Maria
Clinic of Medical Rehabilitation, Medical University of Lodz, 75 Drewnowska Str., Lodz, 91-002, Poland,
Lasers Med Sci. 2014 Sep;29(5):1663-8. doi: 10.1007/s10103-014-1571-y. Epub 2014 Apr 10.
The therapeutic effects of low-power laser radiation of different wavelengths and light doses are well known, but the biochemical mechanism of the interaction of laser light with living cells is not fully understood. We have investigated the effect of MLS (Multiwave Locked System) laser near-infrared irradiation on cell membrane structure, functional properties, and free radical generation using human red blood cells and breast cancer MCF-4 cells. The cells were irradiated with low-intensity MLS near-infrared (simultaneously 808 nm, continuous emission and 905 nm, pulse emission, pulse-wave frequency, 1,000 or 2,000 Hz) laser light at light doses from 0 to 15 J (average power density 212.5 mW/cm(2), spot size was 3.18 cm(2)) at 22 °C, the activity membrane bound acetylcholinesterase, cell stability, anti-oxidative activity, and free radical generation were the parameters used in characterizing the structural and functional changes of the cell. Near-infrared low-intensity laser radiation changed the acetylcholinesterase activity of the red blood cell membrane in a dose-dependent manner: There was a considerable increase of maximal enzymatic rate and Michaelis constant due to changes in the membrane structure. Integral parameters such as erythrocyte stability, membrane lipid peroxidation, or methemoglobin levels remained unchanged. Anti-oxidative capacity of the red blood cells increased after MLS laser irradiation. This irradiation induced a time-dependent increase in free radical generation in MCF-4 cells. Low-intensity near-infrared MLS laser radiation induces free radical generation and changes enzymatic and anti-oxidative activities of cellular components. Free radical generation may be the mechanism of the biomodulative effect of laser radiation.
不同波长和光剂量的低功率激光辐射的治疗效果是众所周知的，但激光与活细胞相互作用的生化机制尚未完全了解。我们使用人红细胞和乳腺癌MCF-4细胞研究了多波锁定系统（MLS）激光近红外照射对细胞膜结构、功能特性和自由基产生的影响。在22°C下，用低强度MLS近红外（同时808nm，连续发射和905nm，脉冲发射，脉冲波频率1000或2000Hz）激光以0至15J的光剂量（平均功率密度212.5mW/cm²，光斑大小为3.18cm²）照射细胞，膜结合乙酰胆碱酯酶活性、细胞稳定性、抗氧化活性和自由基产生是用于表征细胞结构和功能变化的参数。近红外低强度激光辐射以剂量依赖性方式改变红细胞膜的乙酰胆碱酯酶活性：由于膜结构的变化，最大酶促速率和米氏常数显著增加。诸如红细胞稳定性、膜脂质过氧化或高铁血红蛋白水平等整体参数保持不变。MLS激光照射后红细胞的抗氧化能力增强。这种照射诱导MCF-4细胞中自由基产生随时间增加。低强度近红外MLS激光辐射诱导自由基产生并改变细胞成分的酶活性和抗氧化活性。自由基产生可能是激光辐射生物调节作用的机制。
