Liu J, Zhao Y W, Zhao J Q, Xia A D, Jiang L J, Wu S, Ma L, Dong Y Q, Gu Y H
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, China.
J Photochem Photobiol B. 2002 Nov;68(2-3):156-64. doi: 10.1016/s1011-1344(02)00379-2.
The photophysical and photochemical properties of hypocrellins (HA and HB) are examined with two-photon excitations at 800 nm using femtosecond pulses from a Ti:sapphire laser. The two-photon excited fluorescence spectra of HA and HB are very similar to those obtained by one-photon excitation, which may indicate that the two-photon induced photodynamic processes of hypocrellins are similar to one-photon induced photodynamic processes. The two-photon excitation cross sections of HA and HB are measured at 800 nm as about 34.8 x 10(-50) cm(4) s/photon and 21.3 x 10(-50) cm(4) s/photon, respectively. The large two-photon cross sections of both HA and HB, suggest that the hypocrellins can be potential two-photon phototherapeutic agents. As an example for two-photon photodynamic therapy of hypocrellins, we also further examine the cell-damaging effects of HA upon two-photon illumination. Our preliminary results of cell viability test indicate hypocrellins can effectively damage the Hela cells under two-photon illumination.
使用钛宝石激光器产生的飞秒脉冲，在800nm的双光子激发下研究了竹红菌素（HA和HB）的光物理和光化学性质。HA和HB的双光子激发荧光光谱与单光子激发得到的光谱非常相似，这可能表明竹红菌素的双光子诱导光动力过程与单光子诱导光动力过程相似。在800nm处测得HA和HB的双光子激发截面分别约为34.8×10(-50) cm(4) s/光子和21.3×10(-50) cm(4) s/光子。HA和HB都具有较大的双光子截面，表明竹红菌素可能是潜在的双光子光治疗剂。作为竹红菌素双光子光动力治疗的一个例子，我们还进一步研究了HA在双光子照射下对细胞的损伤作用。我们细胞活力测试的初步结果表明，竹红菌素在双光子照射下能有效损伤HeLa细胞。
