Fukui Yoshio
Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611, USA.
Cell Biol Int. 2002;26(11):933-44. doi: 10.1006/cbir.2002.0959.
Dictyostelium serves as an ideal model system for studying the molecular and structural properties of the actomyosin and microtubule systems. This organism also has been the vehicle on which the gene-targeting technique was pioneered. Dictyostelium also represents a small number of organisms whose chemotactic ligand-receptor mechanism has been well characterized. This article reviews recent advances in studies of the actin-based cytoskeletal system in Dictyostelium, focusing on the mechanistic aspects of the amoeboid motion. Special emphasis is placed on the recently identified cell-substrate-anchoring structures 'eupodia', and the measurement of single-cell migration forces. The recent advances in signal transduction cascade is also discussed with relevance to the mechanistics in amoeboid locomotion.
盘基网柄菌是研究肌动球蛋白和微管系统的分子及结构特性的理想模型系统。这种生物体也是基因靶向技术的开创载体。盘基网柄菌还是少数趋化配体 - 受体机制已得到充分表征的生物体之一。本文综述了盘基网柄菌中基于肌动蛋白的细胞骨架系统研究的最新进展，重点关注变形虫运动的机制方面。特别强调了最近发现的细胞 - 底物锚定结构“真足”以及单细胞迁移力的测量。还讨论了信号转导级联反应的最新进展及其与变形虫运动机制的相关性。
