Mathur Surekha, Devaraj Sridevi, Grundy Scott M, Jialal Ishwarlal
Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas 75390-9073, USA.
J Nutr. 2002 Dec;132(12):3663-7. doi: 10.1093/jn/132.12.3663.
Flavonoids and related polyphenolics with antioxidant and anti-inflammatory activities may play a role in the prevention of cardiovascular disease by decreasing oxidative stress and inflammation. We wished to determine the effects of cocoa extract supplementation on markers of oxidative stress and inflammation. Healthy subjects (n = 25) were studied at baseline, after cocoa supplementation (36.9 g of dark chocolate bar and 30.95 g of cocoa powder drink) for 6 wk and after a 6-wk washout period. Fasting blood and early morning urine were collected at the three time points. Two indices of flavonoid intake, total phenols and oxygen radical absorbance capacity of plasma, were measured after an overnight fast. Neither was affected by supplementation. Measures of oxidative stress included copper-catalyzed LDL oxidation kinetics and urinary F(2) isoprostanes. LDL oxidizability was lower after chocolate supplementation as evidenced by a longer lag time (P < 0.05) of conjugated diene formation (101.0 +/- 20.7 min) compared with baseline (91.3 +/- 18.0 min) and washout (96.4 +/- 7.5 min) phases. There was no effect of chocolate on urinary F(2) isoprostane levels or on markers of inflammation including the whole-blood cytokines, interleukin-1 beta, interleukin-6 and tumor necrosis factor-alpha, high sensitivity C-reactive protein and P-selectin. In conclusion, cocoa products supplementation in humans affects LDL oxidizability, but not urinary F(2) isoprostanes or markers of inflammation.
具有抗氧化和抗炎活性的类黄酮及相关多酚类物质可能通过降低氧化应激和炎症反应在预防心血管疾病中发挥作用。我们希望确定补充可可提取物对氧化应激和炎症标志物的影响。对25名健康受试者在基线期、补充可可（36.9克黑巧克力棒和30.95克可可粉饮料）6周后以及6周洗脱期后进行了研究。在这三个时间点采集空腹血液和晨尿。过夜禁食后测量了类黄酮摄入量的两个指标，即总酚和血浆氧自由基吸收能力。两者均不受补充剂的影响。氧化应激的指标包括铜催化的低密度脂蛋白（LDL）氧化动力学和尿F(2)异前列腺素。补充巧克力后LDL的氧化能力降低，共轭二烯形成的延迟时间更长（P < 0.05），补充巧克力后为（101.0 +/- 20.7分钟），与基线期（91.3 +/- 18.0分钟）和洗脱期（96.4 +/- 7.5分钟）相比。巧克力对尿F(2)异前列腺素水平或炎症标志物没有影响，这些标志物包括全血细胞因子、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α、高敏C反应蛋白和P-选择素。总之，在人体中补充可可产品会影响LDL的氧化能力，但不影响尿F(2)异前列腺素或炎症标志物。
