Lefranc Florence, Chaboteaux Carole, Belot Nathalie, Brotchi Jacques, Salmon Isabelle, Kiss Robert
Department of Neurosurgery, Erasmus University Hospital, Brussels, Belgium.
Int J Oncol. 2003 Jan;22(1):213-9.
Gastrin (G17) belongs to the cholecystokinin (CCK) peptide family widely distributed in the brain, and we were the first to show that it significantly modulates the growth and migration features of tumor astyrocytes. Conflictual data have been published as to whether CCKA, CCKB and CCKC receptors are, or are not, present in tumors of the central and peripheral nervous system (CPNS) in general, and in gliomas in particular. In the present study we employed polymerase chain reaction (PCR) on a series of 29 CNPS tumors, including 20 gliomas (17 astrocytic and 3 oligodendroglial tumors), 4 schwannomas and 5 meningiomas to investigate whether RNAs were present or absent in the case of these CCKA, CCKB and CCKC receptors. The presence of the three CCK receptor subtypes was also assayed on three experimental models, i.e. the U373 human glioma, the C6 rat glioma and the 9L rat gliosarcoma. The data show that 9/20 (45%) of the gliomas exhibited RNAs for the CCKB receptor as did the C6 rat glioma, 13/20 (65%) RNAs for the CCKC receptor as did the U373 human glioma and the 9L rat gliosarcoma. Of the 20 gliomas, 17 (85%) expressed RNAs for either the CCKB or the CCKC receptor (or both), a feature which was also observed in the experimental models. One schwannoma and one meningioma exhibited RNAs for the CCKB receptor, while 4/4 schwannomas and 4/5 meningiomas showed RNAs for the CCKC receptor. None of the gliomas, schwannomas or meningiomas exhibited RNAs for the CCKA receptor, which were found in the 9L rat gliosarcoma model only. These data emphasize that 85% of the gliomas under study and 86% (25/29) of the tumors of the central and peripheral nervous system exhibited CCKB and/or CCKC receptors. This therefore suggests an important role for gastrin in the biological development of these tumors.
胃泌素（G17）属于广泛分布于大脑中的胆囊收缩素（CCK）肽家族，我们是首个表明其能显著调节肿瘤星形胶质细胞生长和迁移特性的研究团队。关于CCKA、CCKB和CCKC受体在中枢和外周神经系统（CPNS）肿瘤，尤其是神经胶质瘤中是否存在，已发表了相互矛盾的数据。在本研究中，我们对一系列29个CPNS肿瘤进行了聚合酶链反应（PCR），其中包括20个神经胶质瘤（17个星形细胞瘤和3个少突胶质细胞瘤）、4个神经鞘瘤和5个脑膜瘤，以研究这些CCKA、CCKB和CCKC受体的RNA是否存在。还在三个实验模型，即U373人神经胶质瘤、C6大鼠神经胶质瘤和9L大鼠神经胶质肉瘤上检测了三种CCK受体亚型的存在情况。数据显示，20个神经胶质瘤中有9个（45%）与C6大鼠神经胶质瘤一样，表现出CCKB受体的RNA；13个（65%）与U373人神经胶质瘤和9L大鼠神经胶质肉瘤一样，表现出CCKC受体的RNA。在20个神经胶质瘤中，有17个（85%）表达了CCKB或CCKC受体（或两者）的RNA，这一特征在实验模型中也有观察到。一个神经鞘瘤和一个脑膜瘤表现出CCKB受体的RNA，而4/4的神经鞘瘤和4/5的脑膜瘤表现出CCKC受体的RNA。在神经胶质瘤、神经鞘瘤或脑膜瘤中均未发现CCKA受体的RNA，仅在9L大鼠神经胶质肉瘤模型中发现了该受体的RNA。这些数据强调，所研究的85%的神经胶质瘤和86%（25/29）的中枢和外周神经系统肿瘤表现出CCKB和/或CCKC受体。因此，这表明胃泌素在这些肿瘤的生物学发展中具有重要作用。
