Oikonomou Eftychia, Machado Ana L, Buchfelder Michael, Adams Eric F
School of Health and Life Sciences, Biomedical Chemistry Research Group, Aston University, B4 7ET, Birmingham, UK.
J Neurooncol. 2005 Jul;73(3):199-204. doi: 10.1007/s11060-004-5177-2.
The effect of cholecystokinin (CCK) on cultured human meningioma derived cells was investigated. Exposure of meningioma cells for 6-12 days to CCK-8s (2-200 nM) resulted in a dose dependent stimulation of cell growth to a maximum of 1.1-fold over basal controls. A time course study showed stimulation of cell growth at day 3 followed by increase throughout day 6. The stimulatory effect of CCK on meningioma cell growth was completely abolished by a CCK-B specific receptor antagonist, L-365,260. Reverse-transcription of meningioma-derived RNA into cDNA followed by amplification by the polymerase chain reaction using specific primers for CCK peptide and its CCK-A and/B receptor revealed 100% presence of CCK peptide and CCK-B receptors mRNA whereas CCK-A receptor was expressed in 66% of the meningiomas. These results provide evidence that human meningioma cells possess CCK peptide and its receptors the activation of which leads to increase of cell growth possibly via an autocrine/paracrine mechanism.
研究了胆囊收缩素（CCK）对培养的人脑膜瘤衍生细胞的作用。将脑膜瘤细胞暴露于CCK-8s（2-200 nM）6-12天，导致细胞生长呈剂量依赖性刺激，最高可达基础对照的1.1倍。一项时间进程研究表明，在第3天细胞生长受到刺激，随后在第6天全天增加。CCK对脑膜瘤细胞生长的刺激作用被CCK-B特异性受体拮抗剂L-365,260完全消除。将脑膜瘤衍生的RNA逆转录为cDNA，然后使用CCK肽及其CCK-A和/B受体的特异性引物通过聚合酶链反应进行扩增，结果显示CCK肽和CCK-B受体mRNA的存在率为100%，而CCK-A受体在66%的脑膜瘤中表达。这些结果提供了证据，表明人脑膜瘤细胞拥有CCK肽及其受体，其激活可能通过自分泌/旁分泌机制导致细胞生长增加。
