Gupta Prabodh K, Baloch Zubair W
Department of Pathology, University of Pennsylvania School of Medicine, University of Pennsylvania Health System, Philadelphia, PA 19104, USA.
Semin Diagn Pathol. 2002 Nov;19(4):227-36.
The intraoperative and on-site cytopathology can be successfully performed with a number of smear preparations. Specimen concentration technique is the preferred method. The Romanowsky stain such as Diff Quik used in our laboratory is economical, convenient, reproducible, and quick. This can be at times combined with Ultrafast pap or other rapid stains, which provide good nuclear details. Such evaluations are most valuable in the staging of epithelial tumors and primary diagnosis of a number of central nervous system lesions. On-site and intraoperative diagnoses help triage the specimens for additional studies. This reduces the turn around time and makes the procedure cost-effective and beneficial to the patient. An awareness of the "normal" adjacent structures and familiarity with the cytomorphology of the specimens in comparison to the exfoliative specimens is critical in developing a cytopathology service with high degree of sensitivity and specificity.
通过多种涂片制备方法可成功进行术中及现场细胞病理学检查。标本浓缩技术是首选方法。我们实验室使用的如Diff Quik等罗曼诺夫斯基染色法经济、便捷、可重复且快速。有时可将其与超快速巴氏染色或其他快速染色法相结合，这些方法能提供良好的核细节。此类评估在上皮性肿瘤分期及多种中枢神经系统病变的初步诊断中最具价值。现场及术中诊断有助于对标本进行分类以便进一步研究。这减少了周转时间，使该程序具有成本效益且对患者有益。了解“正常”相邻结构并熟悉与脱落标本相比的标本细胞形态学，对于开展具有高度敏感性和特异性的细胞病理学服务至关重要。
