Biggers John D
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Reprod Biomed Online. 2002;4 Suppl 1:30-8. doi: 10.1016/s1472-6483(12)60009-1.
This review discusses three topics: (i) the 'back to nature' and empirical optimization approaches to the design of chemically defined media for the culture of preimplantation embryos, (ii) the evolution of the simplex optimized family of media, and (iii) adaptation and stress in preimplantation embryos when placed in chemically defined media.
(i) 用于培养植入前胚胎的化学成分确定培养基设计的 “回归自然” 和经验优化方法;(ii) 培养基单纯形优化家族的演变;以及 (iii) 植入前胚胎置于化学成分确定培养基中的适应性和应激反应。