Bjerring Peter, Clement Marc, Heickendorff Lene, Lybecker Hans, Kiernan Mike
Department of Dermatology, University Hospital of Aarhus, Aarhus, Denmark.
J Cosmet Laser Ther. 2002 Jun;4(2):39-43. doi: 10.1080/147641702320602555.
Improvement in the appearance of wrinkles has been observed following exposure to short-pulsed 585 nm laser light. The assumed effect is a specific absorption of light in the blood vessels of the superficial dermis, resulting in release of inflammatory mediators into the interstitium followed by stimulated fibroblast activity. The fibroblasts effectively initiate tissue repair mechanisms, which include enhanced new collagen production.
Quantitative measures of collagen synthesis rate in the skin can be obtained from determinations of the aminoterminal propeptide of type III procollagen level in suction blister fluid using a radioimmunoassay.
A single laser treatment at subpurpura energy level showed that the 585 nm laser source induced an increase of 84% (p < 0.05) in the type III procollagen production rate compared with a non-treated control site. A broadband, pulsed, white light source at 4 J/cm(2) showed no measurable increase, whilst the skin area treated with 7 J/cm(2) increased the procollagen production rate by 17% (NS, p > 0.05). A second treatment 2 weeks later further improved the laser-induced increase in procollagen production rate to 148% (p < 0.05) compared with the control site. The broadband, pulsed, white light-irradiated skin sites showed that at 4 J/cm(2) the procollagen production rate was increased by 21.4% and at 7 J/cm(2) by 32.1% compared with the corresponding non-treated control site (NS, p > 0.05).
Irradiation by the haemoglobin-specific short-pulsed 585 nm laser induced a fivefold increase in procollagen production rate compared with a biologically comparable fluence delivered in a broadband spectrum. An additional treatment after 2 weeks further increased the effect of the short-pulsed 585 nm laser to 148% of the control. Vascular-specific light/tissue interactions seem to play a key role in stimulating skin collagen production.
在暴露于短脉冲585nm激光后，已观察到皱纹外观有所改善。假定的作用机制是，浅表真皮层血管对光的特异性吸收，导致炎症介质释放到间质中，随后刺激成纤维细胞活性。成纤维细胞有效地启动组织修复机制，其中包括增强新胶原蛋白的生成。
通过放射免疫分析法测定抽吸水疱液中III型前胶原氨基末端前肽水平，可获得皮肤中胶原蛋白合成速率的定量指标。
在紫癜能量水平以下进行单次激光治疗显示，与未治疗的对照部位相比，585nm激光源使III型前胶原生成率提高了84%（p<0.05）。4J/cm²的宽带脉冲白光源未显示出可测量的增加，而用7J/cm²治疗的皮肤区域使前胶原生成率提高了17%（无统计学意义，p>0.05）。两周后进行的第二次治疗进一步将激光诱导的前胶原生成率提高至与对照部位相比的148%（p<0.05）。宽带脉冲白光照射的皮肤部位显示，与相应的未治疗对照部位相比，4J/cm²时前胶原生成率提高了21.4%，7J/cm²时提高了32.1%（无统计学意义，p>0.05）。
与宽带光谱中提供的生物学等效能量密度相比，血红蛋白特异性短脉冲585nm激光照射使前胶原生成率提高了五倍。两周后进行的额外治疗进一步将短脉冲585nm激光的效果提高至对照的148%。血管特异性光与组织的相互作用似乎在刺激皮肤胶原蛋白生成中起关键作用。
