Lores B, del Aguila C, Arias C
Instituto de Biotecnología, Universidad de Granada, Granada, España.
Mem Inst Oswaldo Cruz. 2002 Oct;97(7):941-5. doi: 10.1590/s0074-02762002000700003.
In this survey we examined 87 domestic animal stool samples in order to detect the possible presence of microsporidia in animals in close contact with humans in Galicia (NW, Spain). The detection of Enterocytozoon bieneusi spores was confirmed in faecal samples from two dogs and one goat by polymerase chain reaction. None of the positive samples for microsporidia in the staining method were amplified with species-specific primers for Encephalitozoon intestinalis, E. hellem and E. cuniculi. Four rabbits faecal samples reacted with anti-E. cuniculi serum. Our results could indicate the importance of domestic animals as zoonotic reservoirs of microsporidial human infections.
在本次调查中,我们检测了87份家畜粪便样本,以检测西班牙西北部加利西亚地区与人类密切接触的动物体内是否可能存在微孢子虫。通过聚合酶链反应,在两只狗和一只山羊的粪便样本中证实检测到了比氏肠胞微孢子虫孢子。在染色法检测中呈微孢子虫阳性的样本,均未用针对肠脑炎微孢子虫、海伦脑炎微孢子虫和兔脑炎微孢子虫的种特异性引物进行扩增。四份兔粪便样本与抗兔脑炎微孢子虫血清发生反应。我们的结果表明,家畜作为人类微孢子虫感染的人畜共患病储存宿主具有重要意义。