Kamili Saleem, Spelbring John, Krawczynski Krzysztof
Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
J Gastroenterol Hepatol. 2002 Dec;17 Suppl 3:S365-9. doi: 10.1046/j.1440-1746.17.s3.29.x.
: The feasibility of DNA vaccination against hepatitis E in non-human primates has not been evaluated. In the present study a full-length hepatitis E virus (HEV) open reading frame (ORF)2 (Burmese strain) was assembled, cloned, and used for genetic immunization of cynomolgus macaques (cynos), which were subsequently challenged with a heterologous HEV strain (Mexico).
: Four cynos were vaccinated intramuscularly with the HEV ORF2 DNA cassette and one animal was vaccinated with a mock DNA construct.
: Following vaccination anti-HEV antibodies were detected in the four HEV-DNA-vaccinated cynos, but not in the control animal. When challenged, two of the four HEV-DNA-vaccinated cynos were protected against HEV infection and had no elevated alanine aminotransferase activity, viremia, or fecal shedding. The two other DNA-vaccinated animals developed HEV infection and disease.
: These findings demonstrate the feasibility of DNA vaccination for the protection of HEV infection and warrant further studies to explore routes other than intramuscular for induction of a stronger and efficacious immune response.
戊型肝炎DNA疫苗在非人灵长类动物中的可行性尚未得到评估。在本研究中，组装、克隆了全长戊型肝炎病毒（HEV）开放阅读框（ORF）2（缅甸株），并用于食蟹猴的基因免疫，随后用异源HEV株（墨西哥株）对其进行攻击。
四只食蟹猴通过肌肉注射接种HEV ORF2 DNA盒，一只动物接种模拟DNA构建体。
接种疫苗后，在四只接种HEV-DNA的食蟹猴中检测到抗HEV抗体，而在对照动物中未检测到。受到攻击时，四只接种HEV-DNA的食蟹猴中有两只受到保护，未感染HEV，丙氨酸转氨酶活性、病毒血症或粪便排毒均未升高。另外两只接种DNA的动物发生了HEV感染和疾病。
这些发现证明了DNA疫苗预防HEV感染的可行性，值得进一步研究以探索除肌肉注射以外的其他途径来诱导更强效的免疫反应。
