Kamili Saleem, Spelbring John, Carson Dorrie, Krawczynski Krzysztof
Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30084, USA.
J Infect Dis. 2004 Jan 15;189(2):258-64. doi: 10.1086/380801. Epub 2004 Jan 8.
The protective efficacy of a DNA vaccine against hepatitis E virus (HEV) infection was tested in cynomolgus macaques (cynos) vaccinated with a plasmid containing a full-length HEV open-reading frame 2 (ORF2) sequence (Burmese strain) and subsequently challenged with a heterologous strain of HEV (Mexican strain). Cynos administered vaccine by gene gun developed antibodies to HEV (anti-HEV), whereas cynos administered vaccine by intradermal injections and cynos administered a mock DNA construct did not develop anti-HEV. Anti-HEV-positive cynos were protected from HEV infection after challenge with an inoculum that produced infection in the anti-HEV-negative cynos. These results indicate that DNA vaccine with HEV ORF2 administered by gene gun is protective against a heterologous viral challenge.
用含有全长戊型肝炎病毒（HEV）开放阅读框2（ORF2）序列（缅甸株）的质粒对食蟹猴进行接种，随后用异源HEV株（墨西哥株）进行攻击，以此测试DNA疫苗对HEV感染的保护效力。通过基因枪接种疫苗的食蟹猴产生了HEV抗体（抗HEV），而通过皮内注射接种疫苗的食蟹猴以及接种模拟DNA构建体的食蟹猴未产生抗HEV。抗HEV阳性的食蟹猴在用能使抗HEV阴性食蟹猴感染的接种物攻击后受到了保护，未感染HEV。这些结果表明，通过基因枪接种含HEV ORF2的DNA疫苗对异源病毒攻击具有保护作用。
