褪黑素对抗氧化酶基因表达的调节。
Melatonin regulation of antioxidant enzyme gene expression.
作者信息
Mayo J C, Sainz R M, Antoli I, Herrera F, Martin V, Rodriguez C
机构信息
Departamento de Morfologia y Biologia Celular, Facultad de Medicina, c/Julian Claveria s/n, 33006 Oviedo, Austurias, Spain.
出版信息
Cell Mol Life Sci. 2002 Oct;59(10):1706-13. doi: 10.1007/pl00012498.
Antioxidant enzymes (AOEs) are part of the primary cellular defense against free radicals induced by toxins and/or spontaneously formed in cells. Melatonin (MLT) has received much attention in recent years due to its direct free radical scavenging and antioxidant properties. In the present work we report that MLT, at physiological serum concentrations (1 nM), increases the mRNA of both superoxide dismutases (SODs) and glutathione peroxidase (GPx) in two neuronal cell lines. The MLT effect on both SODs and GPx mRNA was mediated by a de novo synthesized protein. MLT alters mRNA stability for Cu-Zn SOD and GPx. Experiments with a short time treatment (pulse action) of MLT suggest that the regulation of AOE gene expression is likely to be receptor mediated, because 1-h treatment with MLT results in the same response as a 24-h treatment.
抗氧化酶（AOEs）是细胞对抗毒素诱导的和/或细胞内自发形成的自由基的主要防御机制的一部分。褪黑素（MLT）近年来因其直接清除自由基和抗氧化特性而备受关注。在本研究中，我们报告生理血清浓度（1 nM）的MLT可增加两种神经元细胞系中超氧化物歧化酶（SODs）和谷胱甘肽过氧化物酶（GPx）的mRNA水平。MLT对SODs和GPx mRNA的影响是由新合成的蛋白质介导的。MLT改变了铜锌超氧化物歧化酶（Cu-Zn SOD）和GPx的mRNA稳定性。MLT短时间处理（脉冲作用）实验表明，AOE基因表达的调节可能是受体介导的，因为MLT 1小时的处理与24小时的处理产生相同的反应。
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