Takenaka Shigeyuki
Fukuoka Institute of Health & Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan.
J Agric Food Chem. 2002 Dec 18;50(26):7515-9. doi: 10.1021/jf025823h.
A new method for the detection and quantification of ethephon residues in fruit and vegetables was developed. The present study indicates that fruit and vegetables require a rapid and simple cleanup step before using gas chromatograph/mass spectrometry. The recovery and precision of the new method were evaluated by spiking the fruit and vegetable samples with 0.01-0.1 microg/g of ethephon. The amount of ethephon residue can be determined with good accuracy (recovery, 78.6-109%; coefficient variation, 2.65-6.41%), and the detection limit, defined as the amount of ethephon equivalent to three standard deviations (SD) of the noise level in observations at the baseline level of the selected ion (m/z 110), was 4 pg. The determination limit, defined as the equivalent to 8 SD of the noise level, was 11 pg. The working range was between 10 and 1000 ng/mL, and the correlation coefficient was 0.999 in the five experiments. Ethephon residues were determined between <2 and 97 ng/g in commercial pineapples from Western Japan.
开发了一种用于检测和定量水果和蔬菜中乙烯利残留的新方法。本研究表明，在使用气相色谱/质谱法之前，水果和蔬菜需要一个快速简单的净化步骤。通过向水果和蔬菜样品中添加0.01 - 0.1微克/克的乙烯利来评估新方法的回收率和精密度。乙烯利残留量能够以良好的准确度测定（回收率为78.6 - 109%；变异系数为2.65 - 6.41%），检测限定义为在选定离子（m/z 110）基线水平下相当于噪声水平三倍标准偏差（SD）的乙烯利量，为4皮克。测定限定义为相当于噪声水平8倍标准偏差，为11皮克。工作范围在10至1000纳克/毫升之间，五次实验中的相关系数为0.999。在日本西部的商业菠萝中，乙烯利残留量测定值在<2至97纳克/克之间。
