Lee Hoi-Seon, Kim Byung-Su, Kim Moo-Key
Research Center for Industrial Development of Biofood Materials and Institute of Agricultural Science & Technology, College of Agriculture, Chonbuk National University, Chonju 561-756, South Korea.
J Agric Food Chem. 2002 Dec 18;50(26):7700-3. doi: 10.1021/jf020751f.
The inhibitory effects of Cinnamomum cassia bark-derived material on nitric oxide (NO) production in RAW 264.7 cells was determined through the evaluation of NO production and expression of inducible nitric oxide and compared to the effects of three commercially available compounds, cinnamyl alcohol, cinnamic acid, and eugenol. The biologically active constituents of C. cassia extract were characterized as trans-cinnamaldehyde by spectral analysis. The inhibitory effects varied with both chemical and concentration used. Potent inhibitory effects of cinnamaldehyde against NO production were 81.5 and 71.7% at 1.0 and 0.5 microg/microL, respectively, and a 41.2% inhibitory effect was revealed at 0.1 microg/microL. However, little or no activity was observed for cinnamic acid and eugenol. Suppression effects of cinnamaldehyde on inducible nitric oxide synthase expression were revealed by Western blot analysis. As a naturally occurring therapeutic agent, trans-cinnamaldehyde could be useful for developing new types of NO inhibitors.
通过评估一氧化氮（NO）的产生以及诱导型一氧化氮的表达，测定了肉桂树皮衍生材料对RAW 264.7细胞中NO产生的抑制作用，并与三种市售化合物肉桂醇、肉桂酸和丁香酚的作用进行了比较。通过光谱分析将肉桂提取物的生物活性成分表征为反式肉桂醛。抑制作用随所用化学物质和浓度的不同而变化。肉桂醛对NO产生的强效抑制作用在1.0和0.5微克/微升时分别为81.5%和71.7%，在0.1微克/微升时显示出41.2%的抑制作用。然而，肉桂酸和丁香酚几乎没有活性或没有活性。通过蛋白质印迹分析揭示了肉桂醛对诱导型一氧化氮合酶表达的抑制作用。作为一种天然存在的治疗剂，反式肉桂醛可能有助于开发新型的NO抑制剂。
