CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, P. R. China.
University of Chinese Academy of Sciences, Beijing, P. R. China.
Prep Biochem Biotechnol. 2021;51(10):1056-1059. doi: 10.1080/10826068.2021.1894444. Epub 2021 Mar 28.
It is a challenge for many researchers to separate volatile compounds. In this study, we introduce a rapid and efficient method of separating target compound from the twigs of by high performance counter-current chromatography. Under the bioassay guidance, the total extract exhibited a potential activity against NO production in RAW 264.7 macrophages and the total extract was further separated by high performance counter-current chromatography. Cinnamaldehyde () was enriched by counter-current chromatography (CCC) with reversed-phase mode using -hexane-ethyl acetate-methanol-water (1:1:1:1,v/v/v/v) as the solvent system. Further identification was achieved by high performance liquid chromatography (HPLC).
对于许多研究人员来说，分离挥发性化合物是一项挑战。在这项研究中，我们介绍了一种从快速高效地分离目标化合物的方法，采用高效逆流色谱法从 的小枝中分离。在生物测定指导下，总提取物对 RAW 264.7 巨噬细胞中 NO 产生表现出潜在的活性，并用高效逆流色谱法进一步分离总提取物。桂皮醛（）采用正相模式的 -己烷-乙酸乙酯-甲醇-水（1:1:1:1，v/v/v/v）作为溶剂系统通过逆流色谱（CCC）富集。通过高效液相色谱法（HPLC）进一步鉴定。
