鸡卵清溶菌酶与7-氯-4-硝基苯并-2-恶唑-1,3-二氮杂茂的反应。II. 修饰位点
Reaction of chicken egg white lysozyme with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole. II. Sites of modification.
作者信息
Aboderin A A, Boedefeld E
出版信息
Biochim Biophys Acta. 1976 Jan 20;420(1):177-86. doi: 10.1016/0005-2795(76)90356-1.
PMID:1247579
Abstract
- Procedure for the isolation of peptides from proteins bearing the chemically labile aromatic ether, O-tyrosyl-4-nitrobenz-2-oxa-1,3-diazole group, is described. 2. The tyrosyl residue reactive towards 7-chloro-4-nitrobenz-2-oxa-1,3-diazole in chicken egg white lysozyme (Aboderin, A. A., Boedefeld, E. and Luisi, P. L., (1973) Biochim. Biophys. Acta 328. 20-30) is tyrosine-23. The amino group in the protein whose reaction with the reagent is dependent on the prior reaction of tyrosine-23 is the epsilon-amino group of lysine-33.
摘要
- 本文描述了从带有化学不稳定芳香醚（O-酪氨酰-4-硝基苯-2-恶唑-1,3-二唑基团）的蛋白质中分离肽段的方法。2. 鸡卵清溶菌酶中对7-氯-4-硝基苯-2-恶唑-1,3-二唑有反应性的酪氨酰残基（阿博德林，A. A.，博德费尔德，E. 和路易西，P. L.，（1973年）《生物化学与生物物理学报》328. 20 - 30）是酪氨酸-23。蛋白质中与该试剂反应依赖于酪氨酸-23先前反应的氨基是赖氨酸-33的ε-氨基。
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