一种用于测量粪便卟啉浓度的高效液相色谱法的精密度和准确性。

Precision and accuracy of a HPLC method for measurement of fecal porphyrin concentrations.

作者信息

Zuijderhoudt Floris M J, Kamphuis Johannes S, Kluitenberg Willem E, Dorresteijn-de Bok Jenneke

机构信息

Department of Clinical Chemistry, Deventer Ziekenhuis, Deventer, The Netherlands.

出版信息

Clin Chem Lab Med. 2002 Oct;40(10):1036-9. doi: 10.1515/CCLM.2002.181.

DOI:10.1515/CCLM.2002.181

PMID:12476945

Abstract

We studied precision and accuracy of a HPLC method for determination of porphyrins in feces. A commercial standard solution appeared to contain less coproporphyrin (15%) than stated by the manufacturer. The between-batch coefficients of variation were often below 15% and were higher than the within-batch coefficients. The precision of porphyrin measurements was not influenced by the type of porphyria. Recoveries of added coproporphyrin and protoporphyrin were 90% and 108%; coefficients of variation were 6% and 19%, respectively.

摘要

我们研究了一种用于测定粪便中卟啉的高效液相色谱法的精密度和准确度。一种市售标准溶液中粪卟啉的含量似乎比制造商宣称的少15%。批次间变异系数通常低于15%，且高于批次内变异系数。卟啉测量的精密度不受卟啉病类型的影响。添加的粪卟啉和原卟啉的回收率分别为90%和108%；变异系数分别为6%和19%。