Christensen N G, Romslo I
Scand J Clin Lab Invest. 1979 May;39(3):223-7. doi: 10.1080/00365517909106097.
When stool copro- and protoporphyrin were quantitated by spectrophotometry, after fractional hydrochloric acid-ether extraction, up to 30% of the protoporphyrins were recovered in the coproporphyrin extract. In disorders with elevated stool protoporphyrin excretion (e.g. erythropoietic protoporphyria), this method therefore may give falsely elevated coproporphyrin values. When the stool porphyrins were determined by high pressure liquid chromatography there was no carry-over of protoporphyrin to coproporphyrin. The recoveries were 82 and 87%, and the coefficients of variation 5.6 and 3.1% for proto- and coproporphyrin, respectively. Moreover, in specimens containing a more complex mixture of porphyrins, a complete separation of the C2-C8 porphyrins and porphyrin isomers was obtained in a single run. High pressure liquid chromatography should therefore be considered the method of choice to obtain a quantitative profile of stool porphyrins in the routine laboratory.
通过分光光度法对粪便粪卟啉和原卟啉进行定量时，经盐酸 - 乙醚分步萃取后，高达30%的原卟啉会在粪卟啉提取物中被回收。因此，在粪便原卟啉排泄升高的疾病（如红细胞生成性原卟啉病）中，该方法可能会给出假性升高的粪卟啉值。当通过高压液相色谱法测定粪便卟啉时，原卟啉不会残留到粪卟啉中。原卟啉和粪卟啉的回收率分别为82%和87%，变异系数分别为5.6%和3.1%。此外，在含有更复杂卟啉混合物的标本中，一次运行就能实现C2 - C8卟啉和卟啉异构体的完全分离。因此，在常规实验室中，高压液相色谱法应被视为获得粪便卟啉定量图谱的首选方法。
