Matejícek David, Klejdus Borivoj, Kubán Vlastimil
Mendel University of Agriculture and Forestry, Department of Chemistry and Biochemistry, Brno, Czech Republic.
J AOAC Int. 2002 Nov-Dec;85(6):1210-6.
A fast, selective, and sensitive liquid chromatographic (LC) method was developed for determination of derivatives of benzoic and cinnamic acids (gallic, protocatechuic, 2,3,4-trihydroxybenzoic, 4-hydroxybenzoic, vanillic, caffeic, syringic, 4-coumaric, ferulic, sinapic, benzoic, 2-coumaric, cinnamic acids, and 4-hydroxybenzaldehyde and vanillin) in soil samples. The method for sample pretreatment is based on temperature-controlled extraction with water (pH 5.6) for 60 min. Extracts were preconcentrated and purified by solid-phase extraction on OASIS HLB sorbent, with subsequent separation and quantification of individual substances by LC with UV diode-array detection. Limits of detection (3 signal-to-noise LODs) better than 65 ng/g (dry weight) and recoveries from 88 to 99% were found for each compound at absorbance 280 nm. The method was used for determination of bioavailable phenolic compounds in different soil samples.
开发了一种快速、选择性和灵敏的液相色谱（LC）方法，用于测定土壤样品中苯甲酸和肉桂酸的衍生物（没食子酸、原儿茶酸、2,3,4-三羟基苯甲酸、4-羟基苯甲酸、香草酸、咖啡酸、丁香酸、4-香豆酸、阿魏酸、芥子酸、苯甲酸、2-香豆酸、肉桂酸、4-羟基苯甲醛和香草醛）。样品预处理方法基于用pH值为5.6的水进行60分钟的控温萃取。提取物通过在OASIS HLB吸附剂上进行固相萃取进行预浓缩和净化，随后通过配备紫外二极管阵列检测的LC对各物质进行分离和定量。在280nm吸光度下，每种化合物的检测限（3倍信噪比检测限）优于65ng/g（干重），回收率为88%至99%。该方法用于测定不同土壤样品中生物可利用的酚类化合物。
