通过实时聚合酶链反应监测骨髓移植受者中活跃的人疱疹病毒6型感染；与通过定性聚合酶链反应检测血浆中病毒DNA的比较。

Monitoring of active HHV-6 infection in bone marrow transplant recipients by real time PCR; comparison to detection of viral DNA in plasma by qualitative PCR.

作者信息

Ihira Masaru, Yoshikawa Tetsushi, Suzuki Kyoko, Ohashi Masahiro, Suga Sadao, Horibe Keizo, Tanaka Naoko, Kimura Hiroshi, Kojima Seiji, Kato Koji, Matsuyama Takaharu, Nishiyama Yukihiro, Asano Yoshizo

机构信息

Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.

出版信息

Microbiol Immunol. 2002;46(10):701-5. doi: 10.1111/j.1348-0421.2002.tb02754.x.

DOI:10.1111/j.1348-0421.2002.tb02754.x

PMID:12477249

Abstract

Twelve (46%) of the 26 patients had human herpesvirus 6 (HHV-6) viremia after bone marrow transplant (BMT). All isolates were recovered from the samples obtained at 2 weeks after BMT. The sensitivity and the specificity of detection of viral DNA in plasma by qualitative polymerase chain reaction (PCR) for monitoring active virus replication were 92% and 97% respectively. Moreover, the positive (85%) and negative (99%) predictive values were also high. The patients with HHV-6 viremia showed a clear peak in HHV-6 DNA in peripheral blood mononuclear cells (PBMCs) at 2 weeks after BMT, which was measured by real time PCR. The virus DNA level in PBMCs between the two groups (patients with viremia and patients without viremia) was statistically different at 2 weeks after BMT (P = 0.033). In patients with HHV-6 viremia, mean HHV-6 DNA copy number was higher in the samples collected at 2 weeks after BMT than the samples collected at any other time period.

摘要

26例患者中有12例（46%）在骨髓移植（BMT）后出现人疱疹病毒6型（HHV-6）病毒血症。所有分离株均从BMT后2周采集的样本中获得。通过定性聚合酶链反应（PCR）检测血浆中病毒DNA以监测病毒活跃复制的敏感性和特异性分别为92%和97%。此外，阳性预测值（85%）和阴性预测值（99%）也很高。HHV-6病毒血症患者在BMT后2周时，外周血单个核细胞（PBMC）中的HHV-6 DNA出现明显峰值，这是通过实时PCR测量的。BMT后2周时，两组（有病毒血症患者和无病毒血症患者）PBMC中的病毒DNA水平存在统计学差异（P = 0.033）。在HHV-6病毒血症患者中，BMT后2周采集的样本中HHV-6 DNA平均拷贝数高于其他任何时间段采集的样本。