Secchiero P, Carrigan D R, Asano Y, Benedetti L, Crowley R W, Komaroff A L, Gallo R C, Lusso P
Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
J Infect Dis. 1995 Feb;171(2):273-80. doi: 10.1093/infdis/171.2.273.
A sensitive and specific polymerase chain reaction method for the detection of human herpesvirus 6 (HHV-6) DNA in serum or plasma has been developed. In total, 157 human serum or plasma samples were studied. HHV-6 DNA was detected in 6 (85.7%) of 7 children with exanthem subitum, 3 (23.1%) of 13 bone marrow transplant (BMT) recipients, 4 (22.2%) of 18 human immunodeficiency virus (HIV)-infected patients, 1 (2.6%) of 39 patients with chronic fatigue syndrome, and none of 37 healthy adults. In the HHV-6-positive BMT recipients, HHV-6 plasma DNA was transiently detected during episodes of fever and respiratory infection. In children with exanthem subitum and in 1 HIV-infected patient, the HHV-6 strains were characterized as variant B, whereas variant A was detected in all other patients. Detection of viral DNA in serum or plasma is a marker of active infection that can be used to investigate the role of HHV-6 in human disease.
已开发出一种灵敏且特异的聚合酶链反应方法，用于检测血清或血浆中的人疱疹病毒6型（HHV-6）DNA。总共研究了157份人血清或血浆样本。在7例幼儿急疹患儿中的6例（85.7%）、13例骨髓移植（BMT）受者中的3例（23.1%）、18例人类免疫缺陷病毒（HIV）感染患者中的4例（22.2%）、39例慢性疲劳综合征患者中的1例（2.6%）检测到HHV-6 DNA，而37例健康成年人中均未检测到。在HHV-6阳性的BMT受者中，在发热和呼吸道感染发作期间短暂检测到HHV-6血浆DNA。在幼儿急疹患儿和1例HIV感染患者中，HHV-6毒株被鉴定为B型变异株，而在所有其他患者中检测到的是A型变异株。血清或血浆中病毒DNA的检测是活动性感染的标志物，可用于研究HHV-6在人类疾病中的作用。
