Soares Marcelo A, De Oliveira Tulio, Brindeiro Rodrigo M, Diaz Ricardo S, Sabino Ester C, Brigido Luís, Pires Ivone L, Morgado Mariza G, Dantas Maria C, Barreira Draurio, Teixeira Paulo R, Cassol Sharon, Tanuri Amilcar
Laboratório de Virologia Molecular, Departamento de Genética, Universidade Federal do Rio de Janeiro, CCS - Bloco A - Cidade Universitária - Ilha do Fundão, 21944-970 Rio de Janeiro, RJ, Brazil.
AIDS. 2003 Jan 3;17(1):11-21. doi: 10.1097/00002030-200301030-00004.
To characterize the subtype C strains of HIV type 1 that circulate in Brazil, especially those originated from the southern part of the country.
One hundred and twelve HIV-1-positive subjects had their plasma viral RNA extracted. Protease (PR) and reverse transcriptase (RT) genomic regions were polymerase chain reaction-amplified and sequenced for subtype determination. Subtype C strains were selected and compared to other strains of this subtype from the database, and specific amino acid signature patterns were searched.
Brazilian subtype C viruses form a very strong monophyletic group when compared to subtype C viruses from other countries and presented specific signature amino acids. Recombinants between subtype C and B viruses have been documented in areas of co-circulation. The incidence of primary PR and RT inhibitor resistance mutations in drug-naïve subjects was observed. An increasing number of secondary resistance mutations was also seen, some of which are characteristic of subtype C-related sequences.
Introduction of subtype C of HIV-1 in Brazil was likely a single event of one or a mixture of similarly related strains. Recombination between subtype C and B viruses is an ongoing process in the country. Primary and secondary drug resistance mutations were observed, although some of the secondary mutations could be associated with subtype C molecular signatures. Subtype-specific polymorphisms of PR and RT sequences found in this subtype C Brazilian variant might influence this emergence and have an impact on HIV treatment and on vaccine development in the country.
鉴定在巴西流行的1型人类免疫缺陷病毒（HIV-1）C亚型毒株，尤其是源自该国南部的毒株。
对112名HIV-1阳性受试者的血浆病毒RNA进行提取。通过聚合酶链反应扩增蛋白酶（PR）和逆转录酶（RT）基因组区域并测序以确定亚型。选择C亚型毒株并与数据库中该亚型的其他毒株进行比较，搜索特定的氨基酸特征模式。
与来自其他国家的C亚型病毒相比，巴西的C亚型病毒形成了一个非常强大的单系群，并呈现出特定的特征氨基酸。在共同流行区域已记录到C亚型和B亚型病毒之间的重组体。观察到初治受试者中主要PR和RT抑制剂耐药突变的发生率。还发现了越来越多的继发性耐药突变，其中一些是C亚型相关序列所特有的。
HIV-1的C亚型在巴西的引入可能是由一种或多种相似相关毒株的单一事件导致。C亚型和B亚型病毒之间的重组在该国是一个持续的过程。观察到了原发性和继发性耐药突变，尽管一些继发性突变可能与C亚型分子特征有关。在这种巴西C亚型变体中发现的PR和RT序列的亚型特异性多态性可能会影响这种情况的出现，并对该国的HIV治疗和疫苗开发产生影响。
