Hamilton James A, Guo Wen, Kamp Frits
Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA 02118, USA.
Mol Cell Biochem. 2002 Oct;239(1-2):17-23.
Defining the mechanism(s) of long-chain fatty acid movement through membranes is vital to understanding whether or not entry of fatty acids into cells can be controlled at the plasma membrane of a typical cell. Is there a protein that acts as gatekeeper, regulating the amount, and possibly the type, of fatty acid that can enter the cell for metabolism? Is the lipid bilayer of the membrane highly permeable to fatty acids, and is the rate of simple diffusion on the time scale of metabolism? We will briefly review efforts to study diffusion in model lipid membranes that are devoid of proteins. We also present new results using dual fluorescence approaches showing that fatty acids diffuse very rapidly across the plasma membrane of the adipocyte.
确定长链脂肪酸通过细胞膜的机制对于理解脂肪酸进入细胞是否能在典型细胞的质膜处受到控制至关重要。是否存在一种蛋白质充当守门人，调节能够进入细胞进行代谢的脂肪酸的数量，甚至可能调节其类型？细胞膜的脂质双层对脂肪酸的渗透性是否很高，以及简单扩散的速率在代谢时间尺度上如何？我们将简要回顾在不含蛋白质的模型脂质膜中研究扩散的相关工作。我们还展示了使用双荧光方法得到的新结果，表明脂肪酸能非常迅速地穿过脂肪细胞质膜。
