Jalava Katri, Hensel Andreas, Szostak Michael, Resch Stephanie, Lubitz Werner
Biotech Innovation Research Development and Consulting (BIRD-C GmbH and CoKEG), Schoenborngasse 12, A-1080, Vienna, Austria.
J Control Release. 2002 Dec 13;85(1-3):17-25. doi: 10.1016/s0168-3659(02)00267-5.
The application of new strategies to develop effective vaccines is essential in modern veterinary medicine. The bacterial ghost system is a novel vaccine delivery system endowed with intrinsic adjuvant properties. Bacterial ghosts are nonliving Gram-negative bacterial cell envelopes devoid of cytoplasmic contents while maintaining their cellular morphology and native surface antigenic structures including bioadhesive properties. They are produced by PhiX174 protein E-mediated lysis of Gram-negative bacteria. The intrinsic adjuvant properties of bacterial ghost preparations enhance immune responses against envelope bound antigens, including T-cell activation and mucosal immunity. Since native and foreign antigens can be expressed in the envelope complex of ghosts before E-mediated lysis, multiple antigens of various origins can be presented to the immune system simultaneously. The advantages of bacterial ghosts include the simplicity of the production method, safety, independence from the cold chain, and versatility as a combination vaccine.
在现代兽医学中，应用新策略开发有效的疫苗至关重要。细菌幽灵系统是一种具有内在佐剂特性的新型疫苗递送系统。细菌幽灵是无生命的革兰氏阴性细菌细胞壁，没有细胞质内容物，同时保持其细胞形态和天然表面抗原结构，包括生物粘附特性。它们是由PhiX174蛋白E介导的革兰氏阴性细菌裂解产生的。细菌幽灵制剂的内在佐剂特性可增强针对包膜结合抗原的免疫反应，包括T细胞活化和黏膜免疫。由于天然和外源抗原可在E介导的裂解之前在幽灵的包膜复合物中表达，因此各种来源的多种抗原可同时呈递给免疫系统。细菌幽灵的优点包括生产方法简单、安全、无需冷链以及作为联合疫苗的多功能性。
