Jalava Katri, Eko Francis O, Riedmann Eva, Lubitz Werner
BIRD-C GmbH & CoKEG, Wien, Austria.
Expert Rev Vaccines. 2003 Feb;2(1):45-51. doi: 10.1586/14760584.2.1.45.
The application of new strategies to develop effective vaccines is essential in modern medicine. The bacterial ghost system is a novel vaccine delivery system endowed with intrinsic adjuvant properties. Bacterial ghosts are nonliving gram-negative bacterial cell envelopes devoid of cytoplasmic contents while maintaining their cellular morphology and native surface antigenic structures including bioadhesive properties. They are produced by PhiX174 protein E-mediated lysis of gram-negative bacteria. The intrinsic adjuvant properties of bacterial ghost preparations enhance immune responses against envelope-bound antigens, including T-cell activation and mucosal immunity. Since native and foreign antigens can be expressed in the envelope complex of ghosts before E-mediated lysis, multiple antigens of various origin can be presented to the immune system simultaneously. In addition, the extended bacterial ghost system represents a platform technology for specific targeting of DNA-encoded antigens to primary antigen-presenting cells. The potency, safety and relatively low production cost of bacterial ghosts offer a significant technical advantage, especially when used as combination vaccines.
应用新策略研发有效的疫苗在现代医学中至关重要。细菌幽灵系统是一种具有内在佐剂特性的新型疫苗递送系统。细菌幽灵是无生命的革兰氏阴性细菌细胞壁包膜，没有细胞质内容物，同时保持其细胞形态和天然表面抗原结构，包括生物粘附特性。它们是通过PhiX174蛋白E介导的革兰氏阴性细菌裂解产生的。细菌幽灵制剂的内在佐剂特性可增强针对包膜结合抗原的免疫反应，包括T细胞活化和黏膜免疫。由于天然和外源抗原可在E介导的裂解之前在幽灵的包膜复合物中表达，因此多种来源的多种抗原可同时呈递给免疫系统。此外，扩展的细菌幽灵系统代表了一种将DNA编码抗原特异性靶向主要抗原呈递细胞的平台技术。细菌幽灵的效力、安全性和相对较低的生产成本提供了显著的技术优势，尤其是用作联合疫苗时。
