Gropper Sareen S, Bader-Crowe D Michele, McAnulty Lisa S, White B Douglas, Keith Robert E
Department of Nutrition and Food Science, Auburn University, Alabama 36849, USA.
J Am Coll Nutr. 2002 Dec;21(6):545-52. doi: 10.1080/07315724.2002.10719253.
Indices of copper status, specifically serum copper and ceruloplasmin concentrations and erythrocyte superoxide dismutase activity, and iron status, including serum ferritin, transferrin receptors, hemoglobin and hematocrit, were studied in 27 college-aged females with adequate iron versus low iron stores.
Serum copper and ceruloplasmin concentrations, erythrocyte superoxide dismutase activity, serum ferritin, transferrin receptors, hemoglobin and hematocrit were studied in 15 females with non-anemic iron depletion before and after five weeks of iron supplementation and in 12 healthy iron-adequate females aged 19 to 28 years.
Mean hemoglobin, hematocrit and ferritin concentrations of the control group (144 +/- 11 g/L, 43 +/- 3% and 38 +/- 15 micro g/L, respectively) were significantly higher than those of the iron depleted group prior to supplementation (134 +/- 9 g/L, 39 +/- 2% and 11 +/- 6 micro g/L, respectively). The serum transferrin receptor to serum ferritin ratio was significantly greater for the iron depleted group prior to supplementation (890 +/- 753) versus the control group (151 +/- 61). Mean serum copper and ceruloplasmin concentrations and erythrocyte superoxide dismutase activity of the iron-adequate control group (20.0 +/- 5.7 micro mol/L, 463 +/- 142 mg/L and 527 +/- 124 U/mL, respectively) were significantly higher than those of the iron depleted group (12.4 +/- 3.8 micro mol/L, 350 +/- 108 mg/L and 353 +/- 186 U/mL, respectively) prior to supplementation. Following iron supplementation, hematocrit and ferritin concentrations of the iron depleted group significantly increased to 42 +/- 3% and 26 +/- 8 micro g/L, respectively. Mean serum transferrin receptor concentrations and the serum transferrin receptor to ferritin ratios significantly decreased in the iron depleted group following supplementation (6.1 +/- 1.6 mg/L to 4.6 +/- 1.5 mg/L and 890 +/- 753 to 198 +/- 114, respectively). Iron supplementation also significantly increased the mean serum copper concentration to 14.2 +/- 5.4 micro mol/L and, in subjects with serum ferritin concentrations </=12 micro g/L, the mean serum ceruloplasmin concentration.
Non-anemic iron depletion characterized by low iron stores is associated with negative impacts on copper status. Iron supplements improved indices of iron status and serum copper and ceruloplasmin concentrations. Whether the diminished serum copper and ceruloplasmin concentrations and superoxide dismutase activity are associated with free radical damage to iron depleted cells requires further investigation.
对27名铁储备充足和铁储备低的大学年龄女性，研究铜状态指标，特别是血清铜和铜蓝蛋白浓度以及红细胞超氧化物歧化酶活性，以及铁状态指标，包括血清铁蛋白、转铁蛋白受体、血红蛋白和血细胞比容。
对15名非贫血性铁缺乏女性在补铁五周前后以及12名年龄在19至28岁的铁储备充足的健康女性，研究血清铜和铜蓝蛋白浓度、红细胞超氧化物歧化酶活性、血清铁蛋白、转铁蛋白受体、血红蛋白和血细胞比容。
对照组的平均血红蛋白、血细胞比容和铁蛋白浓度（分别为144±11 g/L、43±3%和38±15μg/L）显著高于补铁前铁缺乏组（分别为134±9 g/L、39±2%和11±6μg/L）。补铁前铁缺乏组的血清转铁蛋白受体与血清铁蛋白比值（890±753）显著高于对照组（151±61）。铁储备充足的对照组的平均血清铜和铜蓝蛋白浓度以及红细胞超氧化物歧化酶活性（分别为20.0±5.7μmol/L、463±142 mg/L和527±124 U/mL）显著高于补铁前铁缺乏组（分别为12.4±3.8μmol/L、350±108 mg/L和353±186 U/mL）。补铁后，铁缺乏组的血细胞比容和铁蛋白浓度分别显著增至42±3%和26±8μg/L。补铁后铁缺乏组的平均血清转铁蛋白受体浓度以及血清转铁蛋白受体与铁蛋白比值显著降低（分别从6.1±1.6 mg/L降至4.6±1.5 mg/L，从890±753降至198±114）。补铁还使平均血清铜浓度显著增至14.2±5.4μmol/L，并且在血清铁蛋白浓度≤12μg/L的受试者中，平均血清铜蓝蛋白浓度也有所增加。
以铁储备低为特征的非贫血性铁缺乏对铜状态有负面影响。铁补充剂改善了铁状态指标以及血清铜和铜蓝蛋白浓度。血清铜和铜蓝蛋白浓度以及超氧化物歧化酶活性的降低是否与铁缺乏细胞的自由基损伤有关，需要进一步研究。
