Evans Michael J, Van Winkle Laura S, Fanucchi Michelle V, Baker Gregory L, Murphy April E, Nishio Susan J, Schelegle Edward S, Gershwin Laurel J, Sannes Philip L, Plopper Charles G
Department of Anatomy, Physiology, and Cell Biology, Center for Comparative Respiratory Biology and Medicine, School of Veterinary Medicine, University of California, Davis, California 95616, USA.
Lab Invest. 2002 Dec;82(12):1747-54. doi: 10.1097/01.lab.0000043911.94235.f3.
Remodeling of the epithelial basement membrane zone (BMZ) involves increased deposition of collagen, resulting in thickening of the BMZ. The current study focuses on fibroblast growth factor-2 (FGF-2) in the tracheal BMZ in house dust mite allergen (HDMA)-sensitized infant rhesus monkeys, challenged with HDMA at a time when the BMZ is undergoing active postnatal development. To detect structural changes in the BMZ, we measured collagens I, III, and V. To detect changes in the function of the BMZ, we measured immunoreactivity of the heparan sulfate proteoglycan, perlecan, and FGF-2. We found significant thickening of the tracheal BMZ (p < 0.05) with each of these parameters. We also found that all HDMA tracheal samples expressed thin focal areas of the BMZ associated with leukocyte trafficking. These areas were depleted of perlecan and FGF-2; however, increased FGF-2 immunoreactivity was present in the adjacent basal cells. We conclude that basal cells and FGF-2 are involved with significant remodeling of the BMZ in the developing trachea of infant rhesus monkeys exposed to HDMA.
上皮基底膜带（BMZ）的重塑涉及胶原蛋白沉积增加，导致BMZ增厚。本研究聚焦于屋尘螨变应原（HDMA）致敏的幼年恒河猴气管BMZ中的成纤维细胞生长因子2（FGF-2），在BMZ正处于出生后活跃发育阶段时用HDMA进行激发。为检测BMZ的结构变化，我们测量了I型、III型和V型胶原蛋白。为检测BMZ功能的变化，我们测量了硫酸乙酰肝素蛋白聚糖、基底膜聚糖和FGF-2的免疫反应性。我们发现，这些参数中的每一个都导致气管BMZ显著增厚（p < 0.05）。我们还发现，所有HDMA气管样本均表现出与白细胞迁移相关的BMZ薄灶区域。这些区域缺乏基底膜聚糖和FGF-2；然而，相邻基底细胞中FGF-2免疫反应性增加。我们得出结论，基底细胞和FGF-2参与了暴露于HDMA的幼年恒河猴发育中气管BMZ的显著重塑。
