Hamada S, Ishikawa N, Imura T, Torizuka K, Matsumoto Y, Honjo K, Mayumi T
Clin Chim Acta. 1976 Feb 2;66(3):365-70. doi: 10.1016/0009-8981(76)90235-7.
A sensitive method is described for detecting circulating carcinoembryonic antigen (CEA) by combined use of counter-immunoelectrophoresis and intensifiers. Perchloric acid extraction of serum was followed by counter-immunoelectrophoresis against monospecific antiserum to CEA. A precipitin line formed was intensified further by electrophoretic binding of anti-gammaG gamma-globulin and Amido Black staining. Less than 10 ng CEA/ml was detectable. Using this method, a total of 84 sera from patients with or without malignancy was examined. The results obtained were in general agreement with those reported by radioimmunoassay. The method appears to be useful as a diagnostic aid, since it requires neither labeled preparations of purified CEA nor special equipment for assay.
本文描述了一种通过联合使用对流免疫电泳和增强剂来检测循环癌胚抗原（CEA）的灵敏方法。血清经高氯酸提取后，与CEA单特异性抗血清进行对流免疫电泳。形成的沉淀线通过抗γGγ球蛋白的电泳结合和氨基黑染色进一步增强。可检测到的CEA浓度低于10 ng/ml。使用该方法共检测了84例患有或未患有恶性肿瘤患者的血清。所得结果与放射免疫测定法报道的结果基本一致。该方法似乎可作为一种诊断辅助手段，因为它既不需要纯化CEA的标记制剂，也不需要特殊的检测设备。
