Boenisch T, Raam S
Clin Chim Acta. 1975 Feb 8;58(3):195-206. doi: 10.1016/0009-8981(75)90438-6.
Perchloric acid treatment of the carcinoembryonic antigen (CEA) results in subunits of approximate mol. wt 40 000 to 50 000 with different electrophoretic mobilities and amtogenic deficiences as demonstrated by immunoelectrophoresis. An improved radioimmunoassay is described for the quantitation of CEA using rapid ultrafiltration of serum or plasma extracts. The assay was designed to take into account the changes CEA undergoes during perchloric acid extraction. Absorption of antibodies against a crossreacting non-specific antigen from CEA antiserum did not improve the specificity of the radioimmunoassay as was shown by clinical use of the test in almost 1800 patients.
用高氯酸处理癌胚抗原（CEA）会产生分子量约为40000至50000的亚基，这些亚基具有不同的电泳迁移率和免疫原性缺陷，免疫电泳已证实了这一点。本文描述了一种改进的放射免疫测定法，用于通过血清或血浆提取物的快速超滤来定量CEA。该测定法旨在考虑到CEA在高氯酸提取过程中所发生的变化。从CEA抗血清中吸收针对交叉反应性非特异性抗原的抗体，并没有提高放射免疫测定法的特异性，这在近1800例患者的临床检测中得到了证实。
