Ohta Masaru, Hayashi Yasuyuki, Nakashima Asae, Hamada Akira, Tanaka Akira, Nakamura Tatsunosuke, Hayakawa Takahiko
Plantech Research Institute, 1000 Kamoshida-cho, Aoba-ku, Yokohama, 227-0033, Kanagawa, Japan.
FEBS Lett. 2002 Dec 18;532(3):279-82. doi: 10.1016/s0014-5793(02)03679-7.
We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by non-stress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na+/H+ antiporter gene in rice plants significantly improves their salt tolerance.
我们对一个盐敏感型水稻品种（粳稻品种日本晴）进行基因工程改造，使其表达来自盐生植物滨藜（AgNHX1）的液泡型Na⁺/H⁺逆向转运蛋白基因。转基因水稻植株中液泡型Na⁺/H⁺逆向转运蛋白的活性比野生型水稻植株高八倍。在非胁迫处理后的耐盐性试验表明，过表达AgNHX1的转基因植株在300 mM NaCl条件下能存活3天，而野生型水稻植株则不能。这些结果表明，水稻植株中Na⁺/H⁺逆向转运蛋白基因的过表达显著提高了其耐盐性。
