Verma Dheeraj, Singla-Pareek Sneh L, Rajagopal Divya, Reddy M K, Sopory S K
Plant Molecular Biology, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 10067, India.
J Biosci. 2007 Apr;32(3):621-8. doi: 10.1007/s12038-007-0061-9.
Salt stress is an environmental factor that severely impairs plant growth and productivity. We have cloned a novel isoform of a vacuolar Na+/H+ antiporter from Pennisetum glaucum (PgNHX1) that contains 5 transmembrane domains in contrast to AtNHX1 and OsNHX1 which have 9 transmembrane domains. Recently we have shown that PgNHX1 could confer high level of salinity tolerance when overexpressed in Brassica juncea. Here,we report the functional validation of this antiporter in crop plant rice. Overexpression of PgNHX1 conferred high level of salinity tolerance in rice. Transgenic rice plants overexpressing PgNHX1 developed more extensive root system and completed their life cycle by setting flowers and seeds in the presence of 150 mM NaCl. Our data demonstrate the potential of PgNHX1 for imparting enhanced salt tolerance capabilities to salt-sensitive crop plants for growing in high saline areas.
盐胁迫是一种严重损害植物生长和生产力的环境因素。我们从狼尾草中克隆了一种新型液泡Na+/H+逆向转运蛋白异构体（PgNHX1），它含有5个跨膜结构域，而拟南芥液泡Na+/H+逆向转运蛋白（AtNHX1）和水稻液泡Na+/H+逆向转运蛋白（OsNHX1）有9个跨膜结构域。最近我们发现，在芥菜中过表达PgNHX1可使其具有较高的耐盐性。在此，我们报道了这种逆向转运蛋白在作物水稻中的功能验证。PgNHX1的过表达赋予了水稻较高的耐盐性。过表达PgNHX1的转基因水稻植株根系更为发达，并且在150 mM NaCl存在的情况下通过开花结实完成了其生命周期。我们的数据证明了PgNHX1在赋予盐敏感作物增强的耐盐能力以使其能在高盐地区生长方面的潜力。
