Sakakura Chouhei, Simomura Katsumi, Shuichi Kin, Nakase Yuen, Fukuda Ken-ichiro, Takagi Tsuyoshi, Hagiwara Akeo, Ichikawa Yasushi, Nishizuka Itaru, Ishikawa Takashi, Okazaki Yasushi, Yamagishi Hisakazu
Dept. of Gastroenterological Surgery, Kyoto Prefectural University of Medicine.
Gan To Kagaku Ryoho. 2002 Nov;29(12):2271-4.
In this study, we performed a global analysis of the differential gene expression of a gastric cancer cell line established from a primary main tumor (SNU-1) and that of other cell lines established from the metastasis to the peritoneal cavity (KATO-III, SNU-5, SNU-719, MKN45P, HS39). The application of a high-density cDNA microarray method made it possible to analyze the expression of approximately 21,168 genes. Our examinations of KATO-III, SNU-5, SNU-719, MKN45P, and HS39 showed that several genes were up-regulated in addition to expression of sequence tags (ESTs). The analysis revealed altered up-regulation of CD44 (cell adhesion), CEA, 14-3-3, Ubiquitin A and several kinds of ESTs in gastric cancer cells from malignant ascites. We then analyzed these gastric cancer cell lines with Northern blots and observed preferential up-regulation of these selected genes in cells prone to peritoneal dissemination. RT-PCR confirmed that several genes selected by DNA microarray were also overexpressed in clinical samples of malignant ascites. It is therefore considered that these genes may be related to the peritoneal dissemination of gastric cancers. The results of this global gene expression analysis of gastric cancer cells with peritoneal dissemination promise to provide new insights into the study of human gastric cancer peritoneal dissemination.
在本研究中，我们对源自原发性主肿瘤的胃癌细胞系（SNU-1）以及源自腹膜转移灶的其他细胞系（KATO-III、SNU-5、SNU-719、MKN45P、HS39）的差异基因表达进行了全面分析。高密度cDNA微阵列方法的应用使得分析约21,168个基因的表达成为可能。我们对KATO-III、SNU-5、SNU-719、MKN45P和HS39的检测表明，除了序列标签（ESTs）的表达外，还有几个基因上调。分析显示，恶性腹水中的胃癌细胞中CD44（细胞黏附）、CEA、14-3-3、泛素A和几种ESTs的上调发生了改变。然后，我们用Northern印迹法分析了这些胃癌细胞系，并观察到这些选定基因在易于腹膜播散的细胞中优先上调。RT-PCR证实，DNA微阵列选择的几个基因在恶性腹水的临床样本中也过表达。因此认为，这些基因可能与胃癌的腹膜播散有关。对具有腹膜播散的胃癌细胞进行的这种全面基因表达分析结果有望为人类胃癌腹膜播散的研究提供新的见解。
