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丙酮酸羧化酶：三磷酸腺苷镁结合位点的亲和标记

Pyruvate carboxylase: affinity labelling of the magnesium adenosine triphosphate binding site.

作者信息

Easterbrook-Smith S B, Wallace J C, Keech D B

出版信息

Eur J Biochem. 1976 Feb 2;62(1):125-30. doi: 10.1111/j.1432-1033.1976.tb10105.x.

DOI:10.1111/j.1432-1033.1976.tb10105.x
PMID:1248474
Abstract

The 2' , 3'-dialdehyde derivative of ATP (oATP) was prepared by periodate oxidation and on the following criteria was considered to be an effective affinity label. The magnesium complex of this derivative (Mg-oATP2) was shown to ba linear competitive inhibitor with respect to MgATP2-in both the acetyl-CoA-dependent and -independent activities of the enzyme but was a non-competitive inhibitor with respect to bicarbonate, and an uncompetitive inhibitor with respect to pyruvate. Mg-oATP was covalently bound to pyruvate carboxylase by reduction using sodium borohydride with concurrent irreversible inactivation of the enzyme...

摘要

ATP的2'，3'-二醛衍生物（oATP）通过高碘酸盐氧化制备，并基于以下标准被认为是一种有效的亲和标记物。该衍生物的镁配合物（Mg-oATP2）在该酶的乙酰辅酶A依赖性和非依赖性活性方面均显示为MgATP2-的线性竞争性抑制剂，但对于碳酸氢盐是非竞争性抑制剂，对于丙酮酸是反竞争性抑制剂。Mg-oATP通过硼氢化钠还原与丙酮酸羧化酶共价结合，同时使该酶不可逆失活……

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Pyruvate carboxylase: affinity labelling of the magnesium adenosine triphosphate binding site.丙酮酸羧化酶：三磷酸腺苷镁结合位点的亲和标记
Eur J Biochem. 1976 Feb 2;62(1):125-30. doi: 10.1111/j.1432-1033.1976.tb10105.x.
2
Pyruvate carboxylase: affinity labelling of the pyruvate binding site.丙酮酸羧化酶：丙酮酸结合位点的亲和标记
Biochem Biophys Res Commun. 1975 Jul 8;65(1):213-9. doi: 10.1016/s0006-291x(75)80081-7.
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J Biol Chem. 1974 Nov 25;249(22):7057-67.
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Effect of magnesium ion (Mg2+) and the magnesium adenosine triphosphate ion (MgATP2-) on pigeon liver pyruvate carboxylase.镁离子（Mg2+）和三磷酸腺苷镁离子（MgATP2-）对鸽肝丙酮酸羧化酶的影响。
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Prufication and properties of human liver pyruvate carboxylase.人肝脏丙酮酸羧化酶的纯化及性质
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Affinity labelling of rat liver acetyl-CoA carboxylase by a 2',3'-dialdehyde derivative of ATP.用ATP的2',3'-二醛衍生物对大鼠肝脏乙酰辅酶A羧化酶进行亲和标记
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The atypical velocity response by pyruvate carboxylase to increasing concentrations of acetyl-coenzyme A.丙酮酸羧化酶对乙酰辅酶A浓度增加的非典型速度反应。
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Biochem Biophys Res Commun. 1973 Oct 15;54(4):1603-10. doi: 10.1016/0006-291x(73)91170-4.
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