用于诊断公羊精液样本中绵羊布鲁氏菌感染的聚合酶链反应（PCR）检测评估

Evaluation of a PCR test for the diagnosis of Brucella ovis infection in semen samples from rams.

作者信息

Manterola L, Tejero-Garcés A, Ficapal A, Shopayeva G, Blasco J M, Marin C M, López-Goñi I

机构信息

Departamento de Microbiología, Universidad de Navarra, Pamplona 31008, Spain.

出版信息

Vet Microbiol. 2003 Mar 20;92(1-2):65-72. doi: 10.1016/s0378-1135(02)00310-3.

DOI:10.1016/s0378-1135(02)00310-3

PMID:12488071

Abstract

The sensitivity and specificity of a PCR assay with primers derived from the insertion sequence IS6501 was compared with that of bacteriological culture and serological tests for the diagnosis of Brucella ovis infection in rams. No amplifications were detected with DNAs from the strains phylogenetically related to Brucella and from the seven bacterial species considered as the main etiologic agents of epididymitis in rams. In addition, the specificity of the PCR was 100% when testing semen samples from Brucella-free rams. The comparison of the semen culture and PCR results from 192 semen samples tested, showed a proportion of agreement of 0.91 between both tests. The PCR-based test described has sensitivity similar to that of semen culture and could be used as a complementary test for the direct diagnosis of Brucella ovis in semen samples of rams.

摘要

将源自插入序列IS6501的引物用于PCR检测，评估其对公羊绵羊布鲁氏菌感染诊断的敏感性和特异性，并与细菌培养和血清学检测进行比较。与绵羊布鲁氏菌亲缘关系较近的菌株以及被认为是公羊附睾炎主要病原体的7种细菌的DNA均未检测到扩增产物。此外，对公羊的精液样本进行检测时，PCR的特异性为100%。比较192份精液样本的细菌培养和PCR结果，两种检测方法的一致性比例为0.91。所述基于PCR的检测方法的敏感性与精液培养相似，可作为直接诊断公羊精液样本中绵羊布鲁氏菌的补充检测方法。