Bakurdzhiev K, Kokosharov T
Vet Med Nauki. 1976;13(2):78-82.
Semen samples taken from 21 rams with pronounced pathologic changes were studied in a complex way to establish the etiology contributing to the deterioration of the semen quality. The complement-fixation test was applied with blood serum and semen plasma along with the microscopic study of the semen for the excretion of Brucella ovis organisms and a clinical examination. The following results were obtained: 1. The semen plasma of 90.5 per cent of the rams was positive for Brucella ovis. A total of 80.9 per cent were CFT-positive, and the same per cent of the animals were positive for the excretion of Brucella ovis organisms with the semen. The three diagnostic methods give equally positive results in 84.2 per cent of the cases. 2. The clinical examination revealed gross lesions in the genital apparatus in 33.3 per cent of the investigated animals, which coincided with the results obtained through the above-mentioned three methods in 85.9 per cent of the cases. 3. The simultaneous application of the blood serum and semen CFT, the bacteriologic investigation of the semen, and the clinical examination makes it possible to establish the etiology responsible for the defective spermogram of the rams studied.
对21只出现明显病理变化的公羊采集精液样本，进行了全面研究，以确定导致精液质量下降的病因。采用血清和精浆进行补体结合试验，同时对精液进行显微镜检查以检测绵羊布鲁氏菌的排出情况，并进行临床检查。获得了以下结果：1. 90.5%的公羊精浆中绵羊布鲁氏菌呈阳性。总共80.9%的公羊补体结合试验呈阳性，且相同比例的动物精液中排出绵羊布鲁氏菌呈阳性。在84.2%的病例中，这三种诊断方法得出的结果均为阳性。2. 临床检查显示，33.3%的受调查动物生殖器官有明显病变，在85.9%的病例中，这些病变与通过上述三种方法获得的结果一致。3. 同时应用血清和精浆补体结合试验、精液细菌学检查和临床检查，能够确定所研究公羊精液异常的病因。
