与合胞体形成相关的白纹伊蚊蚊细胞系的病毒污染。

Viral contamination of a mosquito cell line, Aedes albopictus, associated with syncytium formation.

作者信息

Hirumi H, Hirumi K, Speyer G, Yunker C E, Thomas L A, Cory J, Sweet B H

出版信息

In Vitro. 1976 Feb;12(2):83-97. doi: 10.1007/BF02796354.

Abstract

Viral contamination associated with syncytium formation in two sbulines of Singh's Aedes albopictus cell cultures was investigated. Electron microscopy of the syncytia revealed the presence of five different types of virus-like particles, which morphologically resembled the parvo-, picorna-, toga-, and orbi-, and bacterial viruses. When a virus-free subline of the A. albopictus cells (SL3) was inoculated with extracts of the syncytium-forming A. albopictus cells, the parvo-, toga-, and orbi-type viral agents were consistently observed. Among these three agents, the togavirus-type agent is most likely responsible for the syncytium induction. Serological examination of the infected cell extract indicated that at least one of three virus-like agents, presumably the togavirus-type agent, was related to Chikungunya. O'nyong-nyong, and Western equine encephalomyelitis viruses (alphaviruses of the Togaviridae), but separable from these.

摘要

对辛格白纹伊蚊细胞培养的两个亚系中与多核细胞形成相关的病毒污染进行了研究。对多核细胞的电子显微镜检查显示存在五种不同类型的病毒样颗粒，其形态类似于细小病毒、小核糖核酸病毒、披膜病毒、圆环病毒和细菌病毒。当用形成多核细胞的白纹伊蚊细胞提取物接种白纹伊蚊细胞的无病毒亚系（SL3）时，始终观察到细小病毒、披膜病毒和圆环病毒型病毒因子。在这三种因子中，披膜病毒型因子最有可能是多核细胞诱导的原因。对感染细胞提取物的血清学检查表明，三种病毒样因子中至少有一种，可能是披膜病毒型因子，与基孔肯雅病毒、奥尼昂尼昂病毒和西部马脑炎病毒（披膜病毒科的甲病毒）有关，但可与这些病毒区分开来。