Cunningham A, Buckley S M, Casals J, Webb S R
J Gen Virol. 1975 Apr;27(1):97-100. doi: 10.1099/0022-1317-27-1-97.
An Aedes albopictus cell line was found contaminated with structures morphologically compatible with an alphavirus. Rapid isolation of a cytopathic virus was effected by combining sonication, concentration with Aquacide II-r, rate zonal sedimentation and subsequent plating of fractions on Vero cells under agar overlay. The virus caused neithedeath nor disease on inoculation into infant and adult mice. It produced a c.p.e. in Vero and BHK 21 cells, and multiplied in Singh's Aedes aegypti cells. The virus was identified serologically as chikungunya by complement-fixation and plaque reduction netralization test. Virus was not detected in a single attempt by these methods in the American Type Culture Collection A. albopicturs line. The presence of chikungunya virus in A. albopictus cells is not easily recognized and may complicate interpretation of experimental results.
发现一株白纹伊蚊细胞系被形态上与甲病毒相符的结构所污染。通过将超声处理、用Aquacide II-r浓缩、速率区带沉降以及随后将各组分在琼脂覆盖下接种到Vero细胞上，实现了细胞病变病毒的快速分离。该病毒接种到幼鼠和成年小鼠体内既未导致死亡也未引发疾病。它在Vero和BHK 21细胞中产生细胞病变效应，并在辛格氏埃及伊蚊细胞中增殖。通过补体结合试验和蚀斑减少中和试验，血清学鉴定该病毒为基孔肯雅病毒。在美国典型培养物保藏中心的白纹伊蚊细胞系中，通过这些方法单次检测均未发现病毒。白纹伊蚊细胞中基孔肯雅病毒的存在不易识别，可能会使实验结果的解释复杂化。
