Honda T, Goshima K, Takeda Y, Sugino Y, Miwatani T
Infect Immun. 1976 Jan;13(1):163-71. doi: 10.1128/iai.13.1.163-171.1976.
Intravenous injection of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus caused rapid death of rats. Studies by electroencephalography and electrocardiography showed that after intravenous injection of the toxin the electroencephalogram remained normal for quite a long time after the heart of the animals had stopped beating. Depression of intraatrial and intraventricular conduction of electrical activation, including atrioventricular block, was observed in electrocardiograms of animals injected with the toxin. The toxin was also found to be toxic to cultured mouse heart cells. When it was added to the medium, the beating rhythm of the cultured heart cells increased temporarily and then soon stopped abruptly. The effect of the toxin on cultured mouse heart cells was blocked by preincubation of the toxin with a ganglioside mixture. From these results it is concluded that the thermostable direct hemolysin (lethal toxin) had cardiotoxic activity, and thus administration of the toxin causes rapid death of animals.
静脉注射副溶血性弧菌产生的耐热直接溶血素（致死毒素）可导致大鼠迅速死亡。脑电图和心电图研究表明，静脉注射毒素后，在动物心脏停止跳动后的相当长一段时间内，脑电图仍保持正常。在注射毒素的动物心电图中观察到心房和心室内电激活传导的抑制，包括房室传导阻滞。还发现该毒素对培养的小鼠心脏细胞有毒性。当将其添加到培养基中时，培养的心脏细胞的跳动节律会暂时增加，然后很快突然停止。毒素与神经节苷脂混合物预孵育可阻断毒素对培养的小鼠心脏细胞的作用。从这些结果可以得出结论，耐热直接溶血素（致死毒素）具有心脏毒性活性，因此给予该毒素会导致动物迅速死亡。
