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通过扫描电子显微镜研究仓鼠气管器官培养中的肺炎支原体。

Mycoplasma pneumoniae in hamster tracheal organ culture studied by scanning electron microscopy.

作者信息

Muse K E, Powell D A, Collier A M

出版信息

Infect Immun. 1976 Jan;13(1):229-37. doi: 10.1128/iai.13.1.229-237.1976.

DOI:10.1128/iai.13.1.229-237.1976
PMID:1248872
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC420599/
Abstract

Hamster tracheal rings in organ culture were inoculated with a virulent strain of Mycoplasma pneumoniae and examined by scanning electron microscopy. A progressive increase in epithelial cell injury was detected from 48 to 96 h post-inoculation and was characterized by apparent loss of the apical portion of ciliated cells. M. pneumoniae attaching to the epithelial cell surfaces could be identified by comparison with the surface morphology of mycoplasmas grown on glass cover slips.

摘要

将器官培养中的仓鼠气管环接种肺炎支原体强毒株，并通过扫描电子显微镜进行检查。在接种后48至96小时检测到上皮细胞损伤逐渐增加，其特征是纤毛细胞顶端部分明显缺失。通过与生长在玻璃盖玻片上的支原体表面形态进行比较，可以识别附着在上皮细胞表面的肺炎支原体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/ab60b4cc0b91/iai00217-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/124573a74b64/iai00217-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/5b8170930660/iai00217-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/c809da065b05/iai00217-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/51811bed444f/iai00217-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/ca5c2e1476f8/iai00217-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/ab60b4cc0b91/iai00217-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/124573a74b64/iai00217-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/5b8170930660/iai00217-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/c809da065b05/iai00217-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/51811bed444f/iai00217-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/ca5c2e1476f8/iai00217-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c9b/420599/ab60b4cc0b91/iai00217-0253-a.jpg

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本文引用的文献

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Relationships Between Mycoplasma pneumoniae and Human Respiratory Epithelium.肺炎支原体与人体呼吸道上皮的关系。
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EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT).肺炎支原体（伊顿氏因子）的实验性感染
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Improvements in epoxy resin embedding methods.环氧树脂包埋方法的改进。
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Ultrastructural observations on cellular and subcellular aspects of experimental Mycoplasma pneumoniae disease.实验性肺炎支原体疾病细胞及亚细胞层面的超微结构观察
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Experimental infection of the respiratory tract with Mycoplasma pneumoniae.肺炎支原体对呼吸道的实验性感染。
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Injury of respiratory epithelium.呼吸道上皮损伤。
Environ Health Perspect. 1980 Apr;35:83-7. doi: 10.1289/ehp.803583.
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The mycoplasmas.支原体。
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Proc Soc Exp Biol Med. 1958 Jul;98(3):489-92. doi: 10.3181/00379727-98-24084.
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Degenerative changes in ciliated cells exfoliating from the bronchial epithelium as a cytologic criterion in the diagnosis of diseases of the lung.支气管上皮纤毛细胞的退行性变化作为肺部疾病诊断的细胞学标准。
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Tissue cultures and mycoplasmas.组织培养与支原体。
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The interrelationship of virulence, cytadsorption, and peroxide formation in Mycoplasma pneumoniae.肺炎支原体中毒力、细胞吸附及过氧化物形成之间的相互关系。
Proc Soc Exp Biol Med. 1969 Sep;131(4):1163-7. doi: 10.3181/00379727-131-34061.
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Mycoplasma pneumoniae in hamster tracheal organ culture: immunofluorescent and electron microscopic studies.仓鼠气管器官培养中的肺炎支原体：免疫荧光和电子显微镜研究。
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Scanning-beam electron microscopy of Mycoplasma pneumoniae.肺炎支原体的扫描束电子显微镜检查
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