Gabridge M G, Agee C C, Cameron A M
J Infect Dis. 1977 Jan;135(1):9-19. doi: 10.1093/infdis/135.1.9.
The morphology of the inner aspect of the adult hamster trachea was examined by scanning electron microscopy. Relatively large patches of unciliated cells were observed in the epithelial layer. The patches, which covered several hundreds to thousands of square microns, were most conspicuous on the ventral surface of the trachea, especially in the middle third. The frequency of these areas of unciliated cells, both isolated and in patches, was much greater in hamsters than in mice, rats, or cats. Greatest ciliation in the hamster trachea was observed over the strip of trachealis muscle between the open ends of the cartilaginous rings. Areas with the heaviest ciliation also had the greatest activity of cellular metabolism, as measured by the tetrazolium reduction assay. The attachment of tritium-labeled cells of Mycoplasma pneumoniae was inversely correlated with extensive ciliation, since the greatest numbers of counts were found on the middle third and ventral regions of the tracheal surface. The results of this study suggest that the regional differences in ciliation of respiratory epithelium in hamsters may influence studies of pathogenesis and isolation of M. pneumoniae and that these differences should therefore be considered and controlled in the experimental design.
通过扫描电子显微镜检查成年仓鼠气管内侧面的形态。在上皮层中观察到相对较大的无纤毛细胞斑块。这些斑块覆盖数百至数千平方微米，在气管腹面最为明显，尤其是在中三分之一处。仓鼠中这些孤立的和斑块状的无纤毛细胞区域的频率比小鼠、大鼠或猫高得多。在软骨环开口端之间的气管肌条上观察到仓鼠气管中纤毛最多。通过四氮唑还原试验测定，纤毛最密集的区域细胞代谢活性也最高。肺炎支原体氚标记细胞的附着与广泛的纤毛形成呈负相关，因为在气管表面的中三分之一和腹侧区域发现的计数最多。本研究结果表明，仓鼠呼吸道上皮纤毛的区域差异可能影响肺炎支原体发病机制的研究和分离，因此在实验设计中应考虑并控制这些差异。
