Gaspirc B, Masera A, Skaleric U
Department of Oral Medicine and Periodontology, Faculty of Medicine, University of Ljubljana, Hrvatski trg 6, 1000 Ljubljana, Slovenia.
Connect Tissue Res. 2002;43(2-3):413-8. doi: 10.1080/03008200290000628.
Localized juvenile periodontitis (LJP) is associated with a destruction of periodontal tissues and the presence of Actinobacillus actinomycetemcomitans (AA). Lipopolysaccharide (LPS) from AA was found to induce a significant macrophage production of nitric oxide (NO). Increased nitric oxide synthase (NOS) activity was found to be negatively correlated with the neutrophil chemotactic response. The aim of this study was to determine the occurrence and distribution of inducible NOS (iNOS) in human gingival tissue from LJP patients. The distribution of iNOS was assessed by monoclonal antibody against iNOS. Cellular markers (CD 3, CD 20, and CD 68) were used to determine the cellular origin of iNOS. The immunostaining revealed the appearance of iNOS in inflamed compared to noninflamed gingival tissues. Macrophages expressed high levels of iNOS that may cause some damage to the periodontal tissues. This study suggests that iNOS activity in macrophages may modify abnormalities of neutrophil function.
局限性青少年牙周炎（LJP）与牙周组织破坏及伴放线放线杆菌（AA）的存在有关。已发现来自AA的脂多糖（LPS）可诱导巨噬细胞大量产生一氧化氮（NO）。一氧化氮合酶（NOS）活性增加与中性粒细胞趋化反应呈负相关。本研究的目的是确定诱导型NOS（iNOS）在LJP患者人牙龈组织中的发生情况和分布。通过抗iNOS单克隆抗体评估iNOS的分布。使用细胞标志物（CD 3、CD 20和CD 68）来确定iNOS的细胞来源。免疫染色显示，与未发炎的牙龈组织相比，发炎的牙龈组织中出现了iNOS。巨噬细胞表达高水平的iNOS，这可能会对牙周组织造成一些损害。本研究表明，巨噬细胞中的iNOS活性可能会改变中性粒细胞功能异常。
